Söllner Stefan, Berkner Silvia, Lipps Georg
Institute of Biochemistry, University of Bayreuth, Universitätstrasse 30, 95447 Bayreuth, Germany.
Extremophiles. 2006 Dec;10(6):629-34. doi: 10.1007/s00792-006-0019-0. Epub 2006 Aug 8.
A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography. The enzyme cuts DNA at the recognition site GCwGC as could be shown by restriction analysis of plasmids and short synthetic duplex DNA. The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C.
通过磷酸纤维素和阳离子交换色谱法纯化了来自冰岛硫化叶菌REN2H1的一种限制性内切核酸酶活性。通过对质粒和短的合成双链DNA进行限制性分析可以表明，该酶在识别位点GCwGC处切割DNA。切割发生在第一个鸟苷碱基之后，并受到5-甲基胞嘧啶甲基化的抑制。该限制性活性对盐敏感，在约70℃时具有最佳活性。
