Grogan D W
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
J Bacteriol. 1989 Dec;171(12):6710-9. doi: 10.1128/jb.171.12.6710-6719.1989.
Though amenable to routine manipulation and a popular subject of molecular genetic and biochemical studies on archaebacteria, the genus Sulfolobus has remained poorly described in phenotypic terms. To delineate their physiological capabilities and diversity, five laboratory strains, including type strains of the described species Sulfolobus acidocaldarius and S. solfataricus, were compared with respect to a variety of growth and biochemical parameters, including component profile of the surface-layer cell wall, inhibitors of growth, growth rate as a function of temperature and pH, and compounds used as sole sources of carbon or nitrogen. Motility and photoregulated production of an orange pigment were detected in all five strains tested. The results provide new criteria for distinguishing Sulfolobus strains as well as potential tools for the physiological and genetic manipulation of these extreme thermophiles.
尽管嗜热栖热菌属易于进行常规操作，并且是古细菌分子遗传学和生物化学研究的热门对象，但在表型方面对其描述仍然很少。为了描述它们的生理能力和多样性，将五个实验室菌株，包括已描述物种嗜酸嗜热栖热菌和嗜热栖热菌的模式菌株，在各种生长和生化参数方面进行了比较，这些参数包括表层细胞壁的成分、生长抑制剂、作为温度和pH函数的生长速率，以及用作唯一碳源或氮源的化合物。在所测试的所有五个菌株中均检测到运动性和橙色色素的光调节产生。这些结果为区分嗜热栖热菌属菌株提供了新的标准，也为这些极端嗜热菌的生理和遗传操作提供了潜在工具。
