一种来自嗜热嗜酸古细菌嗜酸热硫化叶菌的限制性内切酶SuaI。
A restriction endonuclease SuaI from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius.
作者信息
Prangishvili D A, Vashakidze R P, Chelidze M G
出版信息
FEBS Lett. 1985 Nov 11;192(1):57-60. doi: 10.1016/0014-5793(85)80042-9.
PMID:2996942
Abstract
A type II restriction endonuclease (SuaI) has been isolated from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. The enzyme is an isoschizomer of BspRI. It does not cut S. acidocaldarius DNA, as the recognition sequence GGCC in this DNA contains modified nucleotide(s). The enzyme is most active at 60-70 degrees C and is highly thermostable.
摘要
已从嗜热嗜酸古细菌嗜酸热硫化叶菌中分离出一种II型限制性内切酶（SuaI）。该酶是BspRI的同裂酶。它不会切割嗜酸热硫化叶菌的DNA，因为该DNA中的识别序列GGCC含有修饰核苷酸。该酶在60-70摄氏度时活性最高，且具有高度热稳定性。
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