Cerqueira D M, de S Moraes D, Camara G N L, Amaral F A, Oyama C N R, dos Santos M Q C, Martins C R F
Pós-Graduação em Biologia Molecular, Instituto de Biologia, Universidade de Brasília, Brasília, Brazil.
Arch Virol. 2007 Jan;152(1):75-83. doi: 10.1007/s00705-006-0828-6. Epub 2006 Aug 4.
The present study on genetic diversity of human papillomaviruses in women infected by HIV in Brazil describes the frequency, the genotypes, and five new variants of HPV. One hundred fifty cervical smears of HIV-positive women were subjected to cytological examination, and the DNA samples obtained were assayed by MY09/MY11 amplification, followed by RFLP typing. The overall HPV-DNA-positive rate was 42.7%. One hundred twenty-two samples (81.3%) had benign cellular alterations or normal cytological results, and HPV DNA frequency among them was 30.3%. Otherwise, 96.4% of samples with altered cytology were positive for HPV DNA. A high diversity of genotypes was observed. HPVs-16 and 81 were the most prevalent (14.1%) and were followed by HPVs 52, 35, 62, 33, 53, 56, 66, 70, 18, 58, 6b, 11, 31, 39, 40, 61, 71, 32, 54, 59, 67, 68, 85, and 102. Five new variants of the high-risk HPVs 18, 33, 53, 59, and 66 were detected. Possible associations between the detection of HPV genotypes and the cytological classification, HIV viral load, CD4 count, and antiretroviral treatment were also examined. We observed that a high proportion of HIV-infected women are infected with HPV and may carry oncogenic genotypes, even when cytological evaluation shows normal results.
巴西一项关于感染人类免疫缺陷病毒（HIV）女性人乳头瘤病毒（HPV）基因多样性的研究，描述了HPV的感染频率、基因型以及五个新变体。对150例HIV阳性女性的宫颈涂片进行了细胞学检查，并对所获得的DNA样本进行MY09/MY11扩增检测，随后进行限制性片段长度多态性（RFLP）分型。HPV-DNA总体阳性率为42.7%。122份样本（81.3%）有良性细胞改变或细胞学结果正常，其中HPV DNA感染频率为30.3%。否则，96.4%细胞学改变的样本HPV DNA呈阳性。观察到基因型具有高度多样性。HPV-16和81最为常见（14.1%），其次是HPV 52、35、62、33、53、56、66、70、18、58、6b、11、31、39、40、61、71、32、54、59、67、68、85和102。检测到高危型HPV 18、33、53、59和新的六个变体。还研究了HPV基因型检测与细胞学分类、HIV病毒载量、CD4细胞计数和抗逆转录病毒治疗之间的可能关联。我们观察到，即使细胞学评估显示结果正常，仍有很大比例的HIV感染女性感染了HPV，并且可能携带致癌基因型。
