Smirnov Pierre, Lavergne Elise, Gazeau Florence, Lewin Maïté, Boissonnas Alexandre, Doan Bich-Thuy, Gillet Brigitte, Combadière Christophe, Combadière Béhazine, Clément Olivier
Laboratoire de Recherche en Imagerie, Faculté de Médecine Necker, Université Paris Descartes, Paris, France.
Magn Reson Med. 2006 Sep;56(3):498-508. doi: 10.1002/mrm.20996.
The aim of this study was to demonstrate the feasibility of in vivo cell tracking to monitor anticancer cell therapy by means of a high-resolution noninvasive MRI method. Ovalbumin-specific splenocytes (OT-1) labeled with anionic gamma-Fe2O3 superparamagnetic iron oxide (SPIO) nanoparticles were adoptively transferred into C57BL/6 mice with growing ovalbumin-expressing tumors. OT-1 cells were tracked in vivo by 7 T MRI 24, 48, and 72 hr after they were injected. The results showed significant negative enhancement of the spleen at 24 hr, and of the tumor at 48 and 72 hr, after labeled cell injection. This suggests that the lymphocytes initially homed toward the spleen and were then recruited by the tumor. The presence of labeled cells was confirmed in ex vivo by 9.4 T microimaging of tumors and magnetic sorting of spleen cells. These results confirm that MR tracking of lymphocytes is feasible in vivo. This high-resolution imaging method could be used to improve the monitoring of immune cell therapy.
本研究的目的是通过高分辨率无创MRI方法证明体内细胞追踪监测抗癌细胞治疗的可行性。用阴离子γ-Fe2O3超顺磁性氧化铁（SPIO）纳米颗粒标记的卵清蛋白特异性脾细胞（OT-1）被过继转移到患有生长中的表达卵清蛋白肿瘤的C57BL/6小鼠体内。在注射OT-1细胞后24、48和72小时，通过7T MRI对其进行体内追踪。结果显示，注射标记细胞后24小时脾脏出现明显的负性强化，48和72小时肿瘤出现明显的负性强化。这表明淋巴细胞最初归巢至脾脏，然后被肿瘤募集。通过对肿瘤进行9.4T显微成像以及对脾细胞进行磁性分选，在体外证实了标记细胞的存在。这些结果证实淋巴细胞的MR追踪在体内是可行的。这种高分辨率成像方法可用于改进免疫细胞治疗的监测。
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