使用临床1.5特斯拉磁共振成像（MRI）系统对肿瘤浸润淋巴细胞进行体内单细胞检测。

In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system.

作者信息

Smirnov Pierre, Poirier-Quinot Marie, Wilhelm Claire, Lavergne Elise, Ginefri Jean-Christophe, Combadière Béhazine, Clément Olivier, Darrasse Luc, Gazeau Florence

机构信息

Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7-Denis Diderot, Paris, France.

出版信息

Magn Reson Med. 2008 Dec;60(6):1292-7. doi: 10.1002/mrm.21812.

DOI:10.1002/mrm.21812

PMID:19030162

Abstract

We demonstrate the feasibility of detecting individual tumor-infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high-resolution surface coil. Using a recently developed high-temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 microm)(3) at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin-expressing tumors by transferred OVA antigen-specific cytotoxic lymphocytes with low iron load.

摘要

我们通过细胞磁性标记和配备高分辨率表面线圈的1.5特斯拉临床MRI设备，证明了在体内检测单个肿瘤浸润细胞的可行性。使用最近开发的高温超导（HTS）表面线圈，在体外以低至每个细胞0.2 pg铁的磁负荷，在（60微米）³的体素中检测到单个细胞。在体内使用相同的成像方案，以监测低铁负荷的转输OVA抗原特异性细胞毒性淋巴细胞对表达卵清蛋白肿瘤的浸润情况。