由突变的四膜虫端粒酶RNA引起的端粒序列的体内改变和衰老。

In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs.

作者信息

Yu G L, Bradley J D, Attardi L D, Blackburn E H

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley.

出版信息

Nature. 1990 Mar 8;344(6262):126-32. doi: 10.1038/344126a0.

DOI:10.1038/344126a0

PMID:1689810

Abstract

Mutating the CAACCCCAA sequence in the RNA component of telomerase causes the synthesis in vivo of new telomere sequences corresponding to the mutated RNA sequence, demonstrating that the telomerase contains the template for telomere synthesis. These mutations also lead to nuclear and cell division defects, and senescence, establishing an essential role for telomerase in vivo.

摘要

对端粒酶RNA组分中的CAACCCCAA序列进行突变，会导致体内合成与突变RNA序列相对应的新端粒序列，这表明端粒酶包含端粒合成的模板。这些突变还会导致细胞核和细胞分裂缺陷以及衰老，从而确立了端粒酶在体内的重要作用。