Yoshizawa Y, Ishikawa H, Murayama J, Miyashita Y, Yano H, Sato T, Ohtsuka M, Hasegawa S
Institute of Clinical Medicine, University of Tsukuba.
Arerugi. 1993 May;42(5):676-80.
The present study was undertaken to determine the most specific of the 3 serotypes of Trichosporon cutaneum responsible for summer type hypersensitivity pneumonitis and to elucidate the subclass distribution of the antibody response, which is partly dependent on the nature of the causative antigen. Western blot analysis revealed that serotype II (TIMM 1318) is the most specific antigen for summer type hypersensitivity pneumonitis. The subclass distributions of anti-Trichosporon cutaneum IgG antibodies and IgA antibodies in sera and bronchoalveolar lavage fluids (BALF) from 13 patients with summer type hypersensitivity pneumonitis and 10 normal volunteers were measured by biotin-avidin-linked immunosorbent assay. The results were as follows, (1) high levels of IgA2 antibodies were found in BALF, but minute amounts in sera, (2) IgG1 antibodies were the predominant subclass in sera and BALF, (3) three out of 13 cases showed high levels of IgG3 antibodies in sera and BALF associated with low levels of IgG1 antibodies, (4) IgA1 antibodies were found in both sera and BALF. (5) the levels of all antibody subclasses were higher in BALF than in sera. The subclass distributions suggest that the protein components of Trichosporon cutaneum are predominantly immunogen, although polysaccharides may participate as an antigen in the respiratory tract.
本研究旨在确定引起夏季型过敏性肺炎的3种皮癣菌血清型中最具特异性的血清型，并阐明抗体反应的亚类分布，其部分取决于致病抗原的性质。蛋白质印迹分析显示，血清型II（TIMM 1318）是夏季型过敏性肺炎最具特异性的抗原。采用生物素-抗生物素蛋白连接免疫吸附测定法，检测了13例夏季型过敏性肺炎患者和10名正常志愿者血清及支气管肺泡灌洗液（BALF）中抗皮癣菌IgG抗体和IgA抗体的亚类分布。结果如下：（1）BALF中发现高水平的IgA2抗体，但血清中含量极少；（2）IgG1抗体是血清和BALF中的主要亚类；（3）13例中有3例血清和BALF中IgG3抗体水平高，而IgG1抗体水平低；（4）血清和BALF中均发现IgA1抗体；（5）所有抗体亚类在BALF中的水平均高于血清。亚类分布表明，皮癣菌的蛋白质成分主要是免疫原，尽管多糖可能作为抗原参与呼吸道反应。
