Ando M, Sakata T, Yoshida K, Yamasaki H, Araki S, Onoue K, Shinoda T
First Department of Internal Medicine, Kumamoto University Medical School, Japan.
J Allergy Clin Immunol. 1990 Jan;85(1 Pt 1):36-44. doi: 10.1016/0091-6749(90)90218-s.
Inhalation challenge with the culture filtrate-antigens prepared from two strains of different serotype of Trichosporon cutaneum (TIMM 1573, serotype I; TIMM 1318, serotype II) was performed on patients with summer-type hypersensitivity pneumonitis and asymptomatic seropositive family members. Of the 17 patients, 12 were strongly positive, four were mildly so, and one was negative. Interestingly, of the 16 inhalation challenge-positive patients, four reacted to both serotypes I and II, five to serotype I only, and seven to serotype II only. There was a good correlation between the serotype inhalation challenge-positive antigen and that of T. cutaneum isolated from the homes of two patients' homes. A strain of T. cutaneum demonstrating a new serotype was isolated from the homes of two patients, one of whom was negative to both serotypes I and II. Specific antibody activity, lymphocyte proliferative response, and skin reaction to the antigens were also positive, but these findings were not useful to discriminate the inhalation challenge-positive from the inhalation challenge-negative antigen. Neither of the two asymptomatic family members responded. These results indicate that inhalation challenge in patients with summer-type hypersensitivity pneumonitis is provoked by the serotype-related antigen of T. cutaneum, reflecting the sensitization of these patients in their homes.
对夏季型过敏性肺炎患者及无症状血清阳性家庭成员，用从两株不同血清型的皮癣菌（TIMM 1573，血清型I；TIMM 1318，血清型II）制备的培养滤液抗原进行吸入激发试验。17例患者中，12例强阳性，4例弱阳性，1例阴性。有趣的是，16例吸入激发试验阳性患者中，4例对血清型I和II均有反应，5例仅对血清型I有反应，7例仅对血清型II有反应。血清型吸入激发试验阳性抗原与从两名患者家中分离出的皮癣菌的抗原之间存在良好相关性。从两名患者家中分离出一株显示新血清型的皮癣菌，其中一名患者对血清型I和II均呈阴性。对抗原的特异性抗体活性、淋巴细胞增殖反应及皮肤反应也呈阳性，但这些结果无助于区分吸入激发试验阳性抗原和阴性抗原。两名无症状家庭成员均无反应。这些结果表明，夏季型过敏性肺炎患者的吸入激发试验是由皮癣菌的血清型相关抗原引起的，反映了这些患者在家庭中的致敏情况。
