Steif Paul S, Palastro Matthew, Wan Chen-Rei, Baicu Simona, Taylor Michael J, Rabin Yoed
Cell Preserv Technol. 2005 Sep;3(3):184-200. doi: 10.1089/cpt.2005.3.184.
A new imaging device, termed a "cryomacroscope", was used to observe macrofractures in the cryoprotectant cocktails DP6 and VS55. Details of the design and construction of the cryomacroscope were presented in Part I of this report, which focused on describing the apparatus and observations of crystallization. Part I and the current paper (Part II) describe events that occur as 1 mℓ of cryoprotectant contained in a glass vial is cooled from room temperature down to cryogenic temperatures (∼ -135°C). The presence of cracking, as well as patterns in their position and orientation, are found to be dependent on the cooling rate and on the specific cryoprotectant cocktail. Cracks, if present, disappear upon rewarming, although they appear to be sites for later preferential crystallization. Computations which predict temperatures and mechanical stresses are used to explain observations of cracking. In conjunction with these reports, additional photos of cryomacroscopy of vitrification, crystallization, and fracture formation are available at http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm.
一种名为“低温显微镜”的新型成像设备被用于观察冷冻保护剂混合物DP6和VS55中的宏观裂缝。本报告第一部分介绍了低温显微镜的设计和构造细节，该部分重点描述了该仪器以及结晶现象的观察结果。第一部分和本文（第二部分）描述了装有1毫升冷冻保护剂的玻璃瓶从室温冷却至低温温度（约-135°C）时发生的事件。发现裂缝的存在及其位置和方向模式取决于冷却速率和特定的冷冻保护剂混合物。裂缝（如果存在）在重新升温时会消失，尽管它们似乎是后期优先结晶的位点。用于预测温度和机械应力的计算被用于解释裂缝的观察结果。与这些报告相关的是，有关玻璃化、结晶和裂缝形成的低温显微镜的更多照片可在http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm上获取。
