扫描低温显微镜——一种用于低温保存研究的设备原型
The Scanning Cryomacroscope - A Device Prototype for the Study of Cryopreservation.
作者信息
Feig Justin S G, Rabin Yoed
机构信息
Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh PA - 15213, United States.
出版信息
Cryogenics (Guildf). 2014 Jul;62:118-128. doi: 10.1016/j.cryogenics.2014.04.017.
Abstract
A new cryomacroscope prototype-a visualization device for the analysis of cryopreserved biological samples-is presented in the current study. In order to visualize samples larger than the field of view of the optical setup, a scanning mechanism is integrated into the system, which represents a key improvement over previous cryomacroscope prototypes. Another key feature of the new design is in its compatibility with available top-loading controlled-rate cooling chambers, which eliminates the need for a dedicated cooling mechanism. The objective for the current development is to create means to generate a single digital movie of an experimental investigation, with all relevant data overlaid. The visualization capabilities of the scanning cryomacroscope are demonstrated in the current study on the cryoprotective agent dimethyl sulfoxide and the cryoprotective cocktail DP6. Demonstrated effects include glass formation, various regimes of crystallization, thermal contraction, and fracture formation.
摘要
本研究展示了一种新型低温显微镜原型——一种用于分析冷冻保存生物样本的可视化设备。为了可视化大于光学装置视场的样本，系统中集成了扫描机制，这是相对于以前的低温显微镜原型的一项关键改进。新设计的另一个关键特性是它与现有的顶部加载可控速率冷却室兼容，从而无需专门的冷却机制。当前开发的目标是创建一种手段，以生成包含所有相关数据叠加的实验研究的单个数字影片。扫描低温显微镜的可视化能力在当前关于冷冻保护剂二甲基亚砜和冷冻保护剂混合物DP6的研究中得到了证明。展示的效果包括玻璃形成、各种结晶状态、热收缩和裂缝形成。
相似文献
1
The Scanning Cryomacroscope - A Device Prototype for the Study of Cryopreservation.扫描低温显微镜——一种用于低温保存研究的设备原型
Cryogenics (Guildf). 2014 Jul;62:118-128. doi: 10.1016/j.cryogenics.2014.04.017.
2
A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators.一种用于冷冻保存中物理事件可视化的新型 cryomacroscope 设备（III 型），可应用于玻璃化和合成冰调节剂。
Cryobiology. 2013 Dec;67(3):264-73. doi: 10.1016/j.cryobiol.2013.08.005. Epub 2013 Aug 29.
3
Cryomacroscopy of vitrification, Part I: A prototype and experimental observations on the cocktails VS55 and DP6.玻璃化的低温显微镜检查，第一部分：VS55和DP6混合液的原型及实验观察
Cell Preserv Technol. 2005 Sep;3(3):169-183. doi: 10.1089/cpt.2005.3.169.
4
Thermal conductivity of the cryoprotective cocktail DP6 in cryogenic temperatures, in the presence and absence of synthetic ice modulators.在存在和不存在合成冰调节剂的情况下，低温保护剂鸡尾酒DP6在低温下的热导率。
Cryobiology. 2016 Oct;73(2):196-202. doi: 10.1016/j.cryobiol.2016.07.012. Epub 2016 Jul 26.
5
Polarized light scanning cryomacroscopy, part I: Experimental apparatus and observations of vitrification, crystallization, and photoelasticity effects.偏振光扫描低温宏观显微镜，第一部分：实验装置以及玻璃化、结晶和光弹性效应的观察结果
Cryobiology. 2016 Oct;73(2):261-71. doi: 10.1016/j.cryobiol.2016.06.005. Epub 2016 Jun 21.
6
Continuum mechanics analysis of fracture progression in the vitrified cryoprotective agent DP6.玻璃化冷冻保护剂DP6中骨折进展的连续介质力学分析
J Biomech Eng. 2008 Apr;130(2):021006. doi: 10.1115/1.2898716.
7
Cryomacroscopy of vitrification, Part II: Experimental observations and analysis of fracture formation in vitrified VS55 and DP6.玻璃化的低温显微镜检查，第二部分：玻璃化的VS55和DP6中裂缝形成的实验观察与分析
Cell Preserv Technol. 2005 Sep;3(3):184-200. doi: 10.1089/cpt.2005.3.184.
8
Large Thermal Conductivity Differences between the Crystalline and Vitrified States of DMSO with Applications to Cryopreservation.二甲基亚砜结晶态与玻璃态之间的大热导率差异及其在低温保存中的应用
PLoS One. 2015 May 18;10(5):e0125862. doi: 10.1371/journal.pone.0125862. eCollection 2015.
9
Thermal conductivity of cryoprotective agents loaded with nanoparticles, with application to recovery of preserved tissues and organs from cryogenic storage.载纳米颗粒的冷冻保护剂的导热系数，应用于从低温储存中恢复保存的组织和器官。
PLoS One. 2020 Sep 17;15(9):e0238941. doi: 10.1371/journal.pone.0238941. eCollection 2020.
10
Thermal analysis of marginal conditions to facilitate cryopreservation by vitrification using a semi-empirical approach.采用半经验方法分析便于玻璃化冷冻保存的边缘条件的热分析。
Cryobiology. 2019 Dec;91:128-136. doi: 10.1016/j.cryobiol.2019.09.007. Epub 2019 Sep 14.
引用本文的文献
1
Thermomechanical stress analyses of nanowarming-assisted recovery from cryopreservation by vitrification in human heart and rat heart models.纳米升温辅助玻璃化冷冻复苏过程中人心和鼠心模型的热机械应力分析。
PLoS One. 2023 Aug 16;18(8):e0290063. doi: 10.1371/journal.pone.0290063. eCollection 2023.
2
Large surface deformation due to thermo-mechanical effects during cryopreservation by vitrification - mathematical model and experimental validation.玻璃化冷冻保存过程中热-力学效应对大表面变形的影响-数学模型与实验验证。
PLoS One. 2023 Mar 9;18(3):e0282613. doi: 10.1371/journal.pone.0282613. eCollection 2023.
3
Mathematical modeling of surface deformation during vitrification.玻璃化过程中表面变形的数学建模。
Cryobiology. 2021 Oct;102:34-41. doi: 10.1016/j.cryobiol.2021.07.014. Epub 2021 Jul 29.
4
Thermal conductivity of cryoprotective agents loaded with nanoparticles, with application to recovery of preserved tissues and organs from cryogenic storage.载纳米颗粒的冷冻保护剂的导热系数，应用于从低温储存中恢复保存的组织和器官。
PLoS One. 2020 Sep 17;15(9):e0238941. doi: 10.1371/journal.pone.0238941. eCollection 2020.
5
Analysis of polarized-light effects in glass-promoting solutions with applications to cryopreservation and organ banking.玻璃促进溶液中偏光效应分析及其在冷冻保存和器官银行中的应用。
PLoS One. 2018 Jun 18;13(6):e0199155. doi: 10.1371/journal.pone.0199155. eCollection 2018.
6
Thermal conductivity of the cryoprotective cocktail DP6 in cryogenic temperatures, in the presence and absence of synthetic ice modulators.在存在和不存在合成冰调节剂的情况下，低温保护剂鸡尾酒DP6在低温下的热导率。
Cryobiology. 2016 Oct;73(2):196-202. doi: 10.1016/j.cryobiol.2016.07.012. Epub 2016 Jul 26.
7
Polarized light scanning cryomacroscopy, part II: Thermal modeling and analysis of experimental observations.偏振光扫描低温宏观显微镜检查，第二部分：热模型与实验观察分析
Cryobiology. 2016 Oct;73(2):272-81. doi: 10.1016/j.cryobiol.2016.06.004. Epub 2016 Jun 21.
8
Polarized light scanning cryomacroscopy, part I: Experimental apparatus and observations of vitrification, crystallization, and photoelasticity effects.偏振光扫描低温宏观显微镜，第一部分：实验装置以及玻璃化、结晶和光弹性效应的观察结果
Cryobiology. 2016 Oct;73(2):261-71. doi: 10.1016/j.cryobiol.2016.06.005. Epub 2016 Jun 21.
9
Large Thermal Conductivity Differences between the Crystalline and Vitrified States of DMSO with Applications to Cryopreservation.二甲基亚砜结晶态与玻璃态之间的大热导率差异及其在低温保存中的应用
PLoS One. 2015 May 18;10(5):e0125862. doi: 10.1371/journal.pone.0125862. eCollection 2015.
10
On the Effects of Thermal History on the Development and Relaxation of Thermo-Mechanical Stress in Cryopreservation.热历史对冷冻保存中热机械应力发展与松弛的影响
Cryogenics (Guildf). 2014 Nov-Dec;64:86-94. doi: 10.1016/j.cryogenics.2014.09.005.
本文引用的文献
1
A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators.一种用于冷冻保存中物理事件可视化的新型 cryomacroscope 设备（III 型），可应用于玻璃化和合成冰调节剂。
Cryobiology. 2013 Dec;67(3):264-73. doi: 10.1016/j.cryobiol.2013.08.005. Epub 2013 Aug 29.
2
Viscosity of cryoprotective agents near glass transition: a new device, technique, and data on DMSO, DP6, and VS55.玻璃化转变附近的低温保护剂粘度：一种新装置、技术以及关于二甲基亚砜、DP6和VS55的数据。
Exp Mech. 2009 Oct;49(5):663-672. doi: 10.1007/s11340-008-9191-8.
3
Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues.冷冻保护剂鸡尾酒 DP6 与合成冰调节剂在存在和不存在生物组织时的热膨胀。
Cryobiology. 2012 Oct;65(2):117-25. doi: 10.1016/j.cryobiol.2012.04.011. Epub 2012 May 3.
4
Video analysis of osmotic cell response during cryopreservation.冷冻保存过程中渗透细胞响应的视频分析。
Cryobiology. 2012 Jun;64(3):250-60. doi: 10.1016/j.cryobiol.2012.02.008. Epub 2012 Feb 16.
5
Cytotoxicity effects of cryoprotectants as single-component and cocktail vitrification solutions.冷冻保护剂作为单一成分和鸡尾酒玻璃化溶液的细胞毒性作用。
Cryobiology. 2011 Apr;62(2):115-22. doi: 10.1016/j.cryobiol.2011.01.012. Epub 2011 Jan 22.
6
Can thermal expansion differences between cryopreserved tissue and cryoprotective agents alone cause cracking?仅冷冻保存的组织与冷冻保护剂之间的热膨胀差异会导致破裂吗？
Cryo Letters. 2009 Nov-Dec;30(6):414-21.
7
Visualization of intracellular ice formation using high-speed video cryomicroscopy.使用高速视频低温显微镜观察细胞内冰晶形成
Cryobiology. 2009 Feb;58(1):84-95. doi: 10.1016/j.cryobiol.2008.11.003. Epub 2008 Nov 17.
8
Cryopreservation of carotid artery segments via vitrification subject to marginal thermal conditions: correlation of freezing visualization with functional recovery.在边缘热条件下通过玻璃化法对颈动脉段进行冷冻保存：冷冻可视化与功能恢复的相关性
Cryobiology. 2008 Aug;57(1):1-8. doi: 10.1016/j.cryobiol.2008.03.002. Epub 2008 Mar 28.
9
The Effect of Temperature Gradients on Stress Development During Cryopreservation via Vitrification.温度梯度对玻璃化冷冻保存过程中应力发展的影响。
Cell Preserv Technol. 2007;5(2):104-115. doi: 10.1089/cpt.2007.9994.
10
Vitrification of Carotid Artery Segments: An Integrated Study of Thermophysical Events and Functional Recovery Toward Scale-Up for Clinical Applications.颈动脉段的玻璃化：热物理事件与功能恢复的综合研究，旨在向临床应用的扩大规模发展。
Cell Preserv Technol. 2006;4(4):236-244. doi: 10.1089/cpt.2006.9994.
Zotero 插件