Cooper G M, Temin H M
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1027-32. doi: 10.1101/sqb.1974.039.01.118.
We have described an efficient and quantitative assay for infectious DNA of the avian ribodeoxyviruses and have applied this method to study the possible existence of infectious viral DNAs in uninfected cells. Infectious DNA from cells infected with RSV or REV consisted of a single unit of DNA with a specific infectivity of 10(-5)-10(-6). The minimum molecular weight of RSV DNA required for infection of chicken cells was about 6 million, while the minimum molecular weight of infectious REV DNA was about 20 million. This difference may reflect complementation of the RSV DNA by endogenous avian leukosis virus-related DNA in uninfected chicken cells. Uninfected chicken cells do not contain infectious DNA for an REV, nor for a strongly transforming or a nontransforming avian leukosis virus.
我们已经描述了一种针对禽核糖脱氧病毒感染性DNA的高效定量检测方法，并将此方法应用于研究未感染细胞中感染性病毒DNA的可能存在情况。感染劳斯肉瘤病毒（RSV）或禽网状内皮组织增殖症病毒（REV）的细胞中的感染性DNA由单一DNA单位组成，其比感染性为10^(-5)-10^(-6)。感染鸡细胞所需的RSV DNA的最小分子量约为600万，而感染性REV DNA的最小分子量约为2000万。这种差异可能反映了未感染鸡细胞中内源性禽白血病病毒相关DNA对RSV DNA的互补作用。未感染的鸡细胞不含REV的感染性DNA，也不含强转化性或非转化性禽白血病病毒的感染性DNA。
