Cooper G M, Temin H M
J Virol. 1976 Feb;17(2):422-30. doi: 10.1128/JVI.17.2.422-430.1976.
The infectivity of the avian leukosis virus-related genes in the DNA of four genetically distinct types of chicken cells was determined. Infectious DNA of Rous-associated virus-O(RAV-O) was obtained from V- chicken cells which were experimentally infected with RAV-O and from V+tvbs chicken cells, which spontaneously produced RAV-O and were sensitive to exogenous RAV-O infection. However, infectious DNA of RAV-O was not obtained from uninfected V- chicken cells or from V+tvbr chicken cells, which spontaneously produced a low titer of RAV-O but were resistant to exogenous RAV-O infection. No detectable amplification of the RAV-O related DNA sequences in the V+tvbs cells was found by hybridization of RAV-O 125I-labeled RNA to the DNAs of V+tvbs and uninfected V- cells. These results indicate that the endogenous avian leukosis virus-related genes in uninfected V- and V+tvbr cells differ from the RAV-O proviruses in RAV-O-infected V- and V+tvbs cells. The lack of infectivity of the DNA of V+tvbr cells is consistent with the hypothesis that the endogenous RAV-O genome in V+tvbr cells is linked to a cis-acting control element, which results in its inefficient expression.
测定了四种遗传上不同类型鸡细胞DNA中禽白血病病毒相关基因的感染性。劳斯相关病毒-O（RAV-O）的感染性DNA是从经实验感染RAV-O的V-鸡细胞以及自发产生RAV-O且对外源RAV-O感染敏感的V+tvbs鸡细胞中获得的。然而，未感染的V-鸡细胞或自发产生低滴度RAV-O但对外源RAV-O感染有抗性的V+tvbr鸡细胞中未获得RAV-O的感染性DNA。通过将RAV-O 125I标记的RNA与V+tvbs细胞和未感染的V-细胞的DNA杂交，未发现V+tvbs细胞中RAV-O相关DNA序列有可检测到的扩增。这些结果表明，未感染的V-和V+tvbr细胞中的内源性禽白血病病毒相关基因与RAV-O感染的V-和V+tvbs细胞中的RAV-O前病毒不同。V+tvbr细胞DNA缺乏感染性与以下假设一致，即V+tvbr细胞中的内源性RAV-O基因组与一个顺式作用控制元件相连，导致其表达效率低下。
