Nomoto Yukio, Suzuki Teruhisa, Tada Yasuhiro, Kobayashi Ken, Miyake Masao, Hazama Akihiro, Wada Ikuo, Kanemaru Shinichi, Nakamura Tatsuo, Omori Koichi
Department of Otolaryngology, School of Medicine, Fukushima Medical University, 1 Hikarigaoka, Fukushima City 960-1295, Japan.
Ann Otol Rhinol Laryngol. 2006 Jul;115(7):501-6. doi: 10.1177/000348940611500704.
The slowness of epithelialization on the artificial trachea that has been successfully used in humans is a problem. The purpose of this study was to develop a way to regenerate the epithelium on the surface of this artificial trachea.
In an in vitro study, isolated rat tracheal epithelial cells were seeded on a collagenous gel that was stratified on a collagenous sponge. Histologic and immunohistochemical examinations were made. In an in vivo study, we transplanted grafts with green fluorescent protein-positive tracheal epithelial cells onto the tracheal defects of normal rats. At 3, 7, 14, and 30 days after the operation, histologic and immunohistochemical examinations were made.
In the in vitro study, the 3 layers--the epithelium, gel, and sponge--could be observed. The epithelium expressed cytokeratin 14, cytokeratin 18, and occludin. In the in vivo study, the artificial trachea was covered with epithelium at 3 days after operation, and then the epithelium differentiated from single- or double-stratified squamous epithelium into columnar ciliated epithelium. Green fluorescent protein-positive cells were found 3 days after operation.
We believe that the method used in our experiment is an effective way to regenerate the epithelium on the surface of an artificial trachea. With further experimentation, this method should be suitable for clinical application.
已成功应用于人体的人工气管上皮化缓慢是一个问题。本研究的目的是开发一种在这种人工气管表面再生上皮的方法。
在一项体外研究中，将分离的大鼠气管上皮细胞接种在分层于胶原海绵上的胶原凝胶上。进行了组织学和免疫组织化学检查。在一项体内研究中，我们将带有绿色荧光蛋白阳性气管上皮细胞的移植物移植到正常大鼠的气管缺损处。在术后3、7、14和30天进行组织学和免疫组织化学检查。
在体外研究中，可以观察到上皮、凝胶和海绵这三层结构。上皮表达细胞角蛋白14、细胞角蛋白18和闭合蛋白。在体内研究中，术后3天人工气管表面被上皮覆盖，然后上皮从单层或双层鳞状上皮分化为柱状纤毛上皮。术后3天发现绿色荧光蛋白阳性细胞。
我们认为我们实验中使用的方法是在人工气管表面再生上皮的有效方法。通过进一步实验，该方法应适用于临床应用。
