Garbyal Rajendra S, Agarwal Neeta, Kumar Prachi
Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Acta Cytol. 2006 Jul-Aug;50(4):403-6. doi: 10.1159/000325981.
To develop an economical, quick, readily available and simple alternative to May-Grünwald-Giemsa (MGG) stain and so to explore the combination of Leishman and Giemsa stain (LG cocktail).
One wet-fixed and 2 air-dried smears were prepared from 720 cases during the period January 2003-November 2004. The LG cocktail and MGG stain were used on the air-dried smears and Papanicolaou stain in wet-fixed smears. Diagnoses of the cases were made using the LG cocktail-stained smears, and then its diagnostic efficacy was cross-checked with the MGG- and Papanicolaou-stained smears by the same cytopathologist. A comparative study of the LG cocktail and MGG-stained smears was done.
The results achieved with the LG cocktail-stained smears were comparable to or even better than those with the MGG-stained smears, with excellent diagnostic efficacy.
As compared to MGG stain, the 1-step LG cocktail is cheaper, easier to standardize and quicker.
开发一种经济、快速、易于获得且简单的替代迈-格-姬（MGG）染色的方法，从而探索利什曼染色与姬姆萨染色的组合（LG混合染色剂）。
在2003年1月至2004年11月期间，从720例病例中制备了1份湿固定涂片和2份空气干燥涂片。空气干燥涂片使用LG混合染色剂和MGG染色，湿固定涂片使用巴氏染色。使用LG混合染色涂片对病例进行诊断，然后由同一位细胞病理学家用MGG染色涂片和巴氏染色涂片对其诊断效能进行交叉核对。对LG混合染色涂片和MGG染色涂片进行了对比研究。
LG混合染色涂片所获得的结果与MGG染色涂片相当，甚至更好，具有出色的诊断效能。
与MGG染色相比，一步法LG混合染色剂更便宜、更易于标准化且更快。
