Sidhu Supreet K, Ramalingam Karthikeyan, Goyal Sandeep, Poonia Monika, Rajawat Gaurav S, Sharma Nitish
Department of Oral Pathology and Microbiology, Surendera Dental College and Research Institute, Rajasthan University of Health Sciences, Sriganganagar, Rajasthan, India.
J Cytol. 2018 Apr-Jun;35(2):105-109. doi: 10.4103/JOC.JOC_249_16.
This study was planned to compare and evaluate the staining efficacy of Leishman-Giemsa cocktail (LG), Papanicolaou, and Giemsa stain (G) in potentially malignant disorders and malignant lesions.
To evaluate the quality of nuclear and cytoplasmic staining of LG with G, and rapid Papanicolaou stain (Pap) and to compare the total staining efficiency of LG against G and P.
One hundred and eighty participants were studied under three groups - 60 as healthy controls, 60 with potentially malignant disorders, and 60 with malignant lesions; smears were taken thrice from the buccal mucosa. One smear was fixed with Bio-Fix spray and other two smears were allowed to air dry for 2-3 minutes. Then, the ethyl alcohol-fixed smear was stained with Pap and the two other air-dried smears were stained with G and LG stains. Analysis was done using Friedman test and Wilcoxon Signed Rank Test with SPSS Version 15.0.
In the normal group, staining of LG was highly significant ( < 0.001). Among potentially malignant lesions, LG was observed to be highly significant ( < 0.001) when compared with G and was not significant when compared with Pap ( = 0.186). In the malignant group, LG was highly significant ( < 0.001). LG was superior with the highest average staining score of (2.018) than Pap and G.
LG cocktail is a better stain with excellent cytoplasmic and nuclear staining intensity compared to Pap and G stains.
本研究旨在比较和评估利什曼 - 吉姆萨混合染色剂（LG）、巴氏染色法和吉姆萨染色剂（G）在潜在恶性疾病和恶性病变中的染色效果。
评估LG与G、快速巴氏染色法（Pap）的细胞核和细胞质染色质量，并比较LG相对于G和Pap的总染色效率。
180名参与者分为三组进行研究——60名作为健康对照，60名患有潜在恶性疾病，60名患有恶性病变；从颊黏膜采集三次涂片。一张涂片用Bio - Fix喷雾固定，另外两张涂片自然风干2 - 3分钟。然后，用巴氏染色法对乙醇固定的涂片进行染色，另外两张自然风干的涂片分别用G和LG染色剂染色。使用SPSS 15.0软件进行Friedman检验和Wilcoxon符号秩检验分析。
在正常组中，LG染色具有高度显著性（<0.001）。在潜在恶性病变组中，与G相比LG具有高度显著性（<0.001），与Pap相比则无显著性差异（=0.186）。在恶性病变组中，LG具有高度显著性（<0.001）。LG表现更优，平均染色得分最高（2.018），高于Pap和G。
与Pap和G染色剂相比，LG混合染色剂是一种具有出色细胞质和细胞核染色强度的更好的染色剂。
