Asri Rezaei S, Dalir-Naghadeh B
Department of Clinical Sciences, School of Veterinary Medicine, Urmia University, Urmia, P.O. Box 1177, Iran.
Vet Parasitol. 2006 Nov 30;142(1-2):179-86. doi: 10.1016/j.vetpar.2006.05.033. Epub 2006 Aug 9.
To assess the antioxidant status and oxidative stress in bovine theileriosis due to Theileria annulata blood samples were collected from 35 clinically affected cattle referred to Veterinary Teaching Hospital, School of Veterinary Medicine, Urmia University, Urmia, Iran. Complete blood count, piroplasm parasitemia percentage, erythrocyte glutathione peroxidase, superoxide dismutase, catalase and glucose-6-phosphate dehydrogenase activities, malondialdehyde concentration, osmotic fragility test and median corpuscular fragility were determined and the results were compared with those of 50 healthy controls. Of 35 affected cattle, 12 (34.28%) had severe anemia and 23 had mild to moderate anemia and parasitemia varied from 5 to 40%. The activities of erythrocyte glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase were significantly lower (P<0.0001) and the activity of catalase was significantly higher in the affected cattle than in healthy ones (P<0.001). Malondialdehyde concentration in erythrocytes of affected cattle was significantly more than those of healthy cattle (P<0.001). The affected cattle showed increased fragility of erythrocytes, so that median corpuscular fragility (MCF) in affected group was significantly lower than those of healthy group (P<0.0001). Median corpuscular fragility showed a positive correlation with the severity of parasitemia (r=0.81, P<0.0005) and a negative correlation with the activities of GSH-Px (r=-0.78, P<0.0001), SOD (r=-0.71, P<0.0005), catalase (r=-0.53, P<0.018) and G6PD (r=-0.58, P<0.0005). The results of this study suggest that oxidative damage to RBCs may contribute to the pathogenesis of anemia in bovine theileriosis.
为评估环形泰勒虫引起的牛泰勒虫病中的抗氧化状态和氧化应激，从转诊至伊朗乌尔米亚大学兽医学院兽医教学医院的35头临床患病牛采集了血样。测定了全血细胞计数、梨形虫血症百分比、红细胞谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶和葡萄糖-6-磷酸脱氢酶活性、丙二醛浓度、渗透脆性试验和红细胞平均脆性，并将结果与50头健康对照牛的结果进行比较。在35头患病牛中，12头（34.28%）患有严重贫血，23头患有轻度至中度贫血，虫血症从5%至40%不等。患病牛的红细胞谷胱甘肽过氧化物酶、超氧化物歧化酶和葡萄糖-6-磷酸脱氢酶活性显著降低（P<0.0001），而过氧化氢酶活性显著高于健康牛（P<0.001）。患病牛红细胞中的丙二醛浓度显著高于健康牛（P<0.001）。患病牛的红细胞脆性增加，因此患病组的红细胞平均脆性（MCF）显著低于健康组（P<0.0001）。红细胞平均脆性与虫血症严重程度呈正相关（r=0.81，P<0.0005），与谷胱甘肽过氧化物酶（GSH-Px）（r=-0.78，P<0.0001）、超氧化物歧化酶（SOD）（r=-0.71，P<0.0005）、过氧化氢酶（r=-0.53，P<0.018）和葡萄糖-6-磷酸脱氢酶（G6PD）（r=-0.58，P<0.0005）活性呈负相关。本研究结果表明，红细胞的氧化损伤可能在牛泰勒虫病贫血的发病机制中起作用。
