Agina Onyinyechukwu Ada, Cheah Kim Tho, Sayuti Nurul Syahirah Ahmad, Shaari Mohd Rosly, Isa Nur Mahiza Md, Ajat Mokrish, Zamri-Saad Mohd, Mazlan Mazlina, Hamzah Hazilawati
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka 410001, Nigeria.
Animals (Basel). 2021 Jul 29;11(8):2235. doi: 10.3390/ani11082235.
The aim of this study was to measure the serum proinflammatory (IL-12, GM-CSF & IFN-γ) to anti-inflammatory (IL-10, IL-4) cytokine ratio, oxidant (MDA) level and antioxidant enzyme (SOD; GPx) activities after blood parasite infections. The blood and serum samples were obtained from 130 cattle and screened for identity of the infecting blood parasites by conventional PCR. The following blood parasite species were detected: (70/130); (65/130); (32/130); (49/130); (7/130); and (4/130). The GM-CSF/IL-10 ratio showed significantly higher values in all the symptomatic blood parasite infected cattle groups except for symptomatic infected cattle groups. Anti-inflammatory cytokine immune responses were notable findings in symptomatic and asymptomatic cattle infected with and characterized by low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 ( < 0.05). Therefore, high serum GM-CSF:IL:10 in the symptomatic blood parasite infected cattle, low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 ratios in asymptomatic cattle, high MDA level, and increased antioxidant enzyme activities could be useful predictive tools for outcome of natural blood parasite infections in cattle.
本研究的目的是测量血液寄生虫感染后血清促炎细胞因子（白细胞介素 - 12、粒细胞 - 巨噬细胞集落刺激因子和干扰素 - γ）与抗炎细胞因子（白细胞介素 - 10、白细胞介素 - 4）的比值、氧化剂（丙二醛）水平以及抗氧化酶（超氧化物歧化酶；谷胱甘肽过氧化物酶）的活性。从130头牛采集血液和血清样本，并通过常规PCR筛选感染血液寄生虫的种类。检测到以下血液寄生虫种类：（70/130）；（65/130）；（32/130）；（49/130）；（7/130）；以及（4/130）。除有症状的感染牛群外，在所有有症状的血液寄生虫感染牛群中，粒细胞 - 巨噬细胞集落刺激因子/白细胞介素 - 10比值显示出显著更高的值。抗炎细胞因子免疫反应是感染 和 的有症状及无症状牛的显著发现，其特征为血清白细胞介素 - 12:白细胞介素 - 10、干扰素 - γ:白细胞介素 - 10、白细胞介素 - 12:白细胞介素 - 4和干扰素 - γ:白细胞介素 - 4较低（<0.05）。因此，有症状的血液寄生虫感染牛血清中高粒细胞 - 巨噬细胞集落刺激因子:白细胞介素 - 10、无症状牛血清中低白细胞介素 - 12:白细胞介素 - 10、干扰素 - γ:白细胞介素 - 10、白细胞介素 - 12:白细胞介素 - 4和干扰素 - γ:白细胞介素 - 4比值、高丙二醛水平以及抗氧化酶活性增加，可能是预测牛自然血液寄生虫感染结果的有用工具。
