Sabudak Temine, Tareq Hassan Khan Mahmut, Iqbal Choudhary M, Oksuz Sevil
Science and Art Faculty, Department of Chemistry, Trakya University, Edirne, Turkey.
Nat Prod Res. 2006 Jun;20(7):665-70. doi: 10.1080/14786410500196821.
Trifolium balansae (Leguminosae) yielded a phytylester, phytyl-1-hexanoate, three steroids, stigmast-5-ene-3 beta,26-diol, stigmast-5-ene-3-ol and campesterol, and an alcohol, pentacosanol which were reported for the first time from T.balansae. The structures of the isolates were determined by 1D and 2D NMR techniques and MS spectroscopy. Compounds 1-5 were tested for their enzyme tyrosinase activity. While compounds 1 and 5 did not show any inhibition against the enzyme tyrosinase, compounds 2, 3, and 4 exhibited potent inhibition against tyrosinase. Highly potent (IC50 = 2.39 microM) inhibition was found by compound 2, when compared with the standard tyrosinase inhibitors Kojic acid and L-mimosine.
巴朗三叶草（豆科）产生了一种植醇酯，植醇-1-己酸酯，三种甾体化合物，豆甾-5-烯-3β,26-二醇、豆甾-5-烯-3-醇和菜油甾醇，以及一种醇类化合物，二十五烷醇，这些都是首次从巴朗三叶草中报道的。分离物的结构通过一维和二维核磁共振技术以及质谱光谱法确定。对化合物1-5进行了酪氨酸酶活性测试。化合物1和5对酪氨酸酶没有任何抑制作用，而化合物2、3和4对酪氨酸酶表现出强效抑制作用。与标准酪氨酸酶抑制剂曲酸和L-含羞草碱相比，化合物2表现出高效（IC50 = 2.39 microM）抑制作用。
