Muller Daniel, Simeonova Diliana D, Riegel Philippe, Mangenot Sophie, Koechler Sandrine, Lièvremont Didier, Bertin Philippe N, Lett Marie-Claire
Génétique Moléculaire, Génomique, Microbiologie, UMR 7156, CNRS and Université Louis-Pasteur, 28 rue Goethe, 67000 Strasbourg, France.
Laboratoire de Physiopathologie des Infections Bactériennes émergentes et Nosocomiales, Faculté de Médecine, Université Louis-Pasteur, 3 rue Koeberlé, 67000 Strasbourg, France.
Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1765-1769. doi: 10.1099/ijs.0.64308-0.
An arsenite-oxidizing bacterium, designated strain ULPAs1(T), was isolated from industrial sludge heavily contaminated with arsenic. Cells of this isolate were Gram-negative, curved rods, motile by means of a polar flagellum. The strain was positive for oxidase and catalase activities, was able to reduce nitrate to nitrite, used acetate, lactate and peptone as organic carbon sources under aerobic conditions and was able to oxidize arsenite (As[III]) to arsenate (As[V]). 16S rRNA gene sequence analysis and the absence of dodecanoic fatty acids suggested that this strain represents a member of the genus Herminiimonas of the family Oxalobacteraceae, order Burkholderiales in the Betaproteobacteria. Genomic DNA-DNA hybridization between strain ULPAs1(T) and Herminiimonas fonticola S-94(T) and between strain ULPAs1(T) and Herminiimonas aquatilis CCUG 36956(T) revealed levels of relatedness of <10 %, well below the recommended 70 % species cut-off value. Thus, strain ULPAs1(T) (=CCM 7303(T)=DSM 17148(T)=LMG 22961(T)) is the type strain of a novel species of Herminiimonas, for which the name Herminiimonas arsenicoxydans sp. nov. is proposed.
从受砷严重污染的工业污泥中分离出一株亚砷酸盐氧化细菌，命名为菌株ULPAs1(T)。该分离株的细胞为革兰氏阴性、弯曲杆菌，通过极生鞭毛运动。该菌株氧化酶和过氧化氢酶活性呈阳性，能够将硝酸盐还原为亚硝酸盐，在有氧条件下利用乙酸盐、乳酸盐和蛋白胨作为有机碳源，并且能够将亚砷酸盐（As[III]）氧化为砷酸盐（As[V]）。16S rRNA基因序列分析以及不存在十二烷酸表明，该菌株代表β-变形菌纲伯克霍尔德氏菌目草酸杆菌科赫氏单胞菌属的一个成员。菌株ULPAs1(T)与丰泽赫氏单胞菌S-94(T)以及菌株ULPAs1(T)与水生赫氏单胞菌CCUG 36956(T)之间的基因组DNA-DNA杂交显示，相关性水平<10%，远低于推荐的70%的种间界限值。因此，菌株ULPAs1(T)（=CCM 7303(T)=DSM 17148(T)=LMG 22961(T)）是赫氏单胞菌属一个新种的模式菌株，为此提出新种名称砷氧化赫氏单胞菌（Herminiimonas arsenicoxydans sp. nov.）。
