Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):412-417. doi: 10.1099/ijs.0.039073-0. Epub 2012 Mar 23.
A Gram-staining-negative, rod-shaped, non-spore-forming bacterium isolated as a contaminant from a biopharmaceutical process (strain CCUG 53591(T)) was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence similarity data, this strain was clearly allocated to the genus Herminiimonas. Herminiimonas saxobsidens was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (99.9 %), followed by Herminiimonas glaciei (99.6 %) and Herminiimonas arsenicoxydans (98.8 %). Strain ND5, previously reported as H. glaciei, but showing 100 % 16S rRNA gene sequence similarity to strain CCUG 53591(T), was included in the comparative study. Similarities to all other species of the genus Herminiimonas were below 98.0 %. Chemotaxonomic data (major ubiquinone, Q-8; major polar lipids, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; and major fatty acids, C(17 : 0) cyclo, C(19 : 0) cyclo ω8c and C(16 : 0,) with C(10 : 0) 3-OH as hydroxylated fatty acid) supported the affiliation of the isolate to the genus Herminiimonas. DNA-DNA hybridization results (mean values) for strain CCUG 53591(T) with H. saxobsidens CCUG 59860(T) (34 %), H. glaciei DSM 21140(T) (57 %), H. arsenicoxydans DSM 17148(T) (17 %) and Herminiimonas fonticola S-94(T) (11 %) clearly supported the separate taxonomic position of this strain. Strain ND5 showed DNA-DNA similarities of 78, 56 and 52 % to strain CCUG 53591(T), H. glaciei DSM 21140(T) and H. saxobsidens CCUG 59860(T), respectively. Phenotypic differentiation of the isolate from the most closely related species was possible by various features. Hence, strain CCUG 53591(T) represents a novel species, for which the name Herminiimonas contaminans sp. nov. is proposed, with the type strain CCUG 53591(T) ( = CCM 7991(T)). Strain ND5 is a second strain of this species.
一株革兰氏阴性、杆状、不产芽孢的细菌，作为生物制药过程中的污染物从一株菌中分离得到（菌株 CCUG 53591(T)），对其进行了分类学研究。根据 16S rRNA 基因序列相似性数据，该菌株明显属于赫米尼氏菌属。基于 16S rRNA 基因序列相似性（99.9%），赫米尼氏菌属最接近的种是赫米尼氏菌属沙克斯氏菌，紧随其后的是赫米尼氏菌属格陵兰冰菌和赫米尼氏菌属砷氧化菌（分别为 99.6%和 98.8%）。以前报道的菌株 ND5 为赫米尼氏菌属格陵兰冰菌，但与 CCUG 53591(T) 株的 16S rRNA 基因序列相似性为 100%，也被包括在比较研究中。与属内其他种的相似性均低于 98.0%。化学分类学数据（主要醌，Q-8；主要极性脂，磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油；主要脂肪酸，C(17：0) 环、C(19：0) 环 ω8c 和 C(16：0)，C(10：0) 3-OH 为羟基脂肪酸）支持该分离株属于赫米尼氏菌属。CCUG 53591(T) 与赫米尼氏菌属沙克斯氏菌 CCUG 59860(T)（34%）、赫米尼氏菌属格陵兰冰菌 DSM 21140(T)（57%）、赫米尼氏菌属砷氧化菌 DSM 17148(T)（17%）和赫米尼氏菌属泉生菌 S-94(T)（11%）的 DNA-DNA 杂交结果（平均值）清楚地表明该菌株具有独立的分类地位。菌株 ND5 与 CCUG 53591(T)、赫米尼氏菌属格陵兰冰菌 DSM 21140(T) 和赫米尼氏菌属沙克斯氏菌 CCUG 59860(T) 的 DNA-DNA 相似性分别为 78%、56%和 52%。与最密切相关的种相比，分离株的表型分化是可能的，通过各种特征。因此，CCUG 53591(T) 株代表一个新种，命名为赫米尼氏菌属污染菌，模式菌株为 CCUG 53591(T)（=CCM 7991(T)）。菌株 ND5 是该种的第二个菌株。
