对B6.K1和B6.K2 Ped基因同源小鼠植入前胚胎的性别比例分析。
Analysis of the sex ratio in preimplantation embryos from B6.K1 and B6.K2 Ped gene congenic mice.
作者信息
Byrne Michael J, Newmark Judith A, Warner Carol M
机构信息
Department of Biology, Northeastern University, 134 Mugar Hall, Boston, MA 02115, USA.
出版信息
J Assist Reprod Genet. 2006 Jul-Aug;23(7-8):321-8. doi: 10.1007/s10815-006-9046-0. Epub 2006 Aug 11.
PURPOSE
The mouse preimplantation embryo development (Ped) gene product, Qa-2, which is the homolog of human HLA-G, influences the rate of preimplantation embryonic development and overall reproductive success. The sex ratio in preimplantation embryos from Ped gene congenic mice was examined in order to determine whether embryo sex is a confounding factor in the control of the rate of preimplantation development.
METHODS
B6.K1 (Ped slow) and B6.K2 (Ped fast) congenic mice differ only in the absence (B6.K1) or presence (B6.K2) of the genes encoding Qa-2 protein. We analyzed the sex of B6.K1 (n=221) and B6.K2 (n=260) preimplantation embryos by using Real-Time PCR with primers specific for the X and Y chromosomes.
RESULTS
We found that there was no statistically significant difference in the ratio of male to female preimplantation embryos in either strain.
CONCLUSIONS
We conclude that the sex of the embryos is not a confounding factor that affects the Ped gene control of the rate of preimplantation development. Therefore, the Ped gene is entirely responsible for mediating the faster development of B6.K2 embryos compared to B6.K1 embryos.
目的
小鼠着床前胚胎发育（Ped）基因产物Qa-2是人类HLA-G的同源物，它影响着床前胚胎发育的速率和整体繁殖成功率。为了确定胚胎性别是否是着床前发育速率控制中的一个混杂因素，我们对Ped基因同源小鼠着床前胚胎的性别比例进行了检测。
方法
B6.K1（Ped慢）和B6.K2（Ped快）同源小鼠仅在编码Qa-2蛋白的基因的缺失（B6.K1）或存在（B6.K2）方面存在差异。我们使用针对X和Y染色体的特异性引物通过实时PCR分析了B6.K1（n = 221）和B6.K2（n = 260）着床前胚胎的性别。
结果
我们发现，在这两个品系中，着床前胚胎的雌雄比例均无统计学上的显著差异。
结论
我们得出结论，胚胎性别不是影响Ped基因对着床前发育速率控制的混杂因素。因此，与B6.K1胚胎相比，Ped基因完全负责介导B6.K2胚胎的更快发育。
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