Chen Hsin-I, Tsai Chen-Der, Wang Hann-Tsong, Hwang Shiaw-Min
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 30062, Taiwan.
Cryobiology. 2006 Oct;53(2):283-7. doi: 10.1016/j.cryobiol.2006.06.002. Epub 2006 Aug 10.
Cryopreservation is one of the fundamental techniques in life science. To preserve the viability of cells and tissues, many researchers use plastic cryogenic vials and immerse them into liquid nitrogen for long-term storage. However, the non-sterile liquid nitrogen usually infiltrates into the vials and may cause a high rate of microbial contamination, and even some explosive incidents upon retrieval. To prevent these drawbacks while retaining the benefit of constant ultra-low temperature in submerged liquid nitrogen, we used a heat-sealable membrane to cover the upper portion of vials. After heat-sealing, the vials were completely free of liquid nitrogen penetration in the submerging test. Moreover, the sealing process did not affect the cell viability. This modified protocol provides an easy and efficient tool to ensure the integrity of biospecimens in long-term storage without interfering with existing cryobox storage systems.
冷冻保存是生命科学中的一项基本技术。为了保持细胞和组织的活力，许多研究人员使用塑料低温冻存管，并将其浸入液氮中进行长期储存。然而，未灭菌的液氮通常会渗入冻存管，可能导致高微生物污染率，甚至在取出时引发一些爆炸事故。为了在保留液氮浸没时恒定超低温优势的同时防止这些缺点，我们使用了一种可热封的膜覆盖冻存管上部。热封后，在浸没测试中冻存管完全没有液氮渗入。此外，密封过程不影响细胞活力。这种改进的方案提供了一种简单有效的工具，可确保生物样本在长期储存中的完整性，而不会干扰现有的低温储存系统。
