Wang Feng, Huang Wei
Department of Chemistry, Zaozhuang University, Shandong 277160, PR China.
J Pharm Biomed Anal. 2007 Jan 4;43(1):393-8. doi: 10.1016/j.jpba.2006.07.007. Epub 2006 Aug 10.
Curcumin (C21H20O6, CU) is a natural antioxidant, which is considered to be a very useful compound in health matters, and is employed in the treatment of cardiovascular and arthritic illnesses. It is found that the fluorescence intensity of Eu3+-tryptophan (Trp) can be greatly quenched by curcumin in the buffer of pH 7.7. Under optimum conditions, the quenched intensity of fluorescence is in proportion to the concentration of curcumin in the range of 1.0x10(-8) to 1.2x10(-4) mol L(-1). The detection limit (S/N=3) is 9.0x10(-10) mol L(-1). The synthetic and actual samples are satisfactorily determined. In addition, the interaction mechanism is also investigated.
姜黄素（C21H20O6，CU）是一种天然抗氧化剂，被认为是在健康问题方面非常有用的化合物，可用于治疗心血管疾病和关节炎。研究发现，在pH 7.7的缓冲溶液中，姜黄素能显著猝灭Eu3 + -色氨酸（Trp）的荧光强度。在最佳条件下，荧光猝灭强度与姜黄素浓度在1.0×10（-8）至1.2×10（-4）mol L（-1）范围内成正比。检测限（S/N = 3）为9.0×10（-10）mol L（-1）。该方法对合成样品和实际样品的测定结果令人满意。此外，还研究了相互作用机理。
