Hofmann G E, Anderson T L
Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk.
Am J Obstet Gynecol. 1990 Mar;162(3):837-41. doi: 10.1016/0002-9378(90)91020-d.
Epidermal growth factor receptor was localized immunohistochemically during implantation (gestational days 7 through 14) in the rabbit. During early implantation (days 7 through 9), immunostaining was observed in the cytoplasm and on the surface of the syncytiotrophoblastic knobs. By day 8, immunoreactivity was most pronounced in knobs interacting directly with uterine epithelium but was noted in nonknob cytotrophoblast as well. Fetal membranes, identified by day 10, were stained intensely on all days examined. In later gestation the trophoblast was stained intensely on the surface and in the cytoplasm, with some staining over nuclei. No appreciable changes in the staining pattern were observed after day 10. Uterine epithelium demonstrated epidermal growth factor receptor staining first on the antimesometrial (day 7) and then the paraplacental epidermal (day 9). After day 10, most staining was at the embryo-maternal interphase. These data suggest that the embryo and uterine epithelia can receive signals from their environment during and after implantation through the epidermal growth factor receptor.
采用免疫组织化学方法对兔着床期（妊娠第7至14天）的表皮生长因子受体进行定位。在着床早期（第7至9天），在合体滋养层结节的细胞质和表面观察到免疫染色。到第8天，与子宫上皮直接相互作用的结节中免疫反应最为明显，但在非结节细胞滋养层中也有发现。在第10天识别出的胎膜，在所有检查的日子里都有强烈染色。在妊娠后期，滋养层在表面和细胞质中有强烈染色，细胞核上也有一些染色。第10天后未观察到染色模式有明显变化。子宫上皮首先在反系膜（第7天），然后在胎盘旁表皮（第9天）显示表皮生长因子受体染色。第10天后，大部分染色位于胚胎 - 母体界面。这些数据表明，胚胎和子宫上皮在着床期间及之后可通过表皮生长因子受体接收来自其环境的信号。
