Bergh P A, Anderson T L, Hofmann G E
Department of Obstetrics and Gynecology, Mount Sinai Medical Center, New York, NY 10029.
Am J Obstet Gynecol. 1991 Apr;164(4):1127-31. doi: 10.1016/0002-9378(91)90600-v.
Gonadotropin-releasing hormone was localized immunohistochemically during implantation (gestational days 6 to 14) in the New Zealand White rabbit. During early implantation (days 7 to 9), intense gonadotropin-releasing hormone immunostaining was localized predominantly to the cytoplasm of the nonknob cytotrophoblast with light to moderate staining in the cytoplasm of the syncytiotrophoblast (knob). In later gestation, light to moderate staining of the cytoplasm of the trophoblast at the true placental site was detected. No appreciable change in staining was noted after day 10. Fetal membranes, identified after day 10, showed intense and unchanging immunostaining for gonadotropin-releasing hormone. Obplacental giant cells showed light to moderate nuclear and cytoplasmic gonadotropin-releasing hormone immunostaining. Light to moderate gonadotropin-releasing hormone immunostaining was also noted in the cytoplasm of uterine epithelium and glands. We conclude that immunoreactive gonadotropin-releasing hormone is present in the cytotrophoblast at the time of the earliest embryo-uterine interactions and may play a significant role in implantation and embryo survival.
采用免疫组织化学方法对新西兰白兔着床期（妊娠第6至14天）的促性腺激素释放激素进行定位。在着床早期（第7至9天），促性腺激素释放激素免疫染色强烈，主要定位于非结节细胞滋养层的细胞质，合体滋养层（结节）的细胞质有轻度至中度染色。在妊娠后期，在真正胎盘部位的滋养层细胞质中检测到轻度至中度染色。第10天后染色未见明显变化。第10天后识别出的胎膜显示促性腺激素释放激素免疫染色强烈且不变。胎盘外巨细胞显示轻度至中度的促性腺激素释放激素核染色和细胞质染色。子宫上皮和腺体的细胞质中也可见轻度至中度的促性腺激素释放激素免疫染色。我们得出结论，在最早的胚胎-子宫相互作用时，细胞滋养层中存在免疫反应性促性腺激素释放激素，其可能在着床和胚胎存活中发挥重要作用。
