Hofmann G E, Horowitz G M, Scott R T, Navot D
Department of Obstetrics and Gynecology, Mount Sinai Medical Center, New York, New York 10029.
J Clin Endocrinol Metab. 1993 Mar;76(3):781-5. doi: 10.1210/jcem.76.3.7680357.
Epidermal growth factor (EGF) and its receptor (EGF-R) have been demonstrated in human implantation sites. Transforming growth factor-alpha (TGF-alpha), a protein with extensive sequence homology to EGF and with equal affinity for the EGF-R, was localized immunohistochemically in early intrauterine and ectopic pregnancies. Within the same experiments, TGF-alpha immunostaining was more intense in ectopic than intrauterine pregnancies. In both groups, TGF-alpha immunostaining was moderate to intense in the syncytiotrophoblast (ST), light to moderate in the cytotrophoblast (CT), and moderate to intense in intermediate trophoblast (IT). In ST, TGF-alpha immunostaining localized to the cytoplasm and plasma membranes, including microvilli. No nuclear associated TGF-alpha was noted in ST. In CT, differential TGF-alpha immunostaining was noted between the villous and nonvillous CT. Villous CT demonstrated light to absent cytoplasmic TGF-alpha immunostaining with intense nuclear staining. In contrast, nonvillous CT revealed moderate to intense cytoplasmic staining without demonstrable nuclear staining. These results demonstrate the presence of immunoreactive TGF-alpha in all forms of trophoblast. The known presence of the EGF-R suggests an autocrine/paracrine role for TGF-alpha during human implantation.
表皮生长因子（EGF）及其受体（EGF-R）已在人类着床部位得到证实。转化生长因子-α（TGF-α）是一种与EGF具有广泛序列同源性且对EGF-R具有同等亲和力的蛋白质，通过免疫组织化学方法定位在早期宫内妊娠和异位妊娠中。在同一实验中，异位妊娠中TGF-α免疫染色比宫内妊娠更强烈。在两组中，TGF-α免疫染色在合体滋养层（ST）中为中度至强烈，在细胞滋养层（CT）中为轻度至中度，在中间滋养层（IT）中为中度至强烈。在ST中，TGF-α免疫染色定位于细胞质和质膜，包括微绒毛。在ST中未发现与核相关的TGF-α。在CT中，绒毛CT和非绒毛CT之间存在TGF-α免疫染色差异。绒毛CT显示细胞质TGF-α免疫染色轻度至无，核染色强烈。相反，非绒毛CT显示中度至强烈的细胞质染色，无明显核染色。这些结果证明了所有形式的滋养层中均存在免疫反应性TGF-α。已知EGF-R的存在表明TGF-α在人类着床过程中具有自分泌/旁分泌作用。
