Gelazonia L, Japaridze N, Svanidze I
Institute of Physiology, Georgian Academy of Sciences, Tbilisi, Georgia.
Georgian Med News. 2006 Jun(135):126-8.
In the present study the influences of toluene intoxication on quantity of the pyramidal and granular cells in hippocampus was analysed in young and adult rats. An inhalation route of intoxication was used in our experiments - animal inhaled toluene until attaining a sidewise laying position. This procedure was performed in the closed glass container, in which air was beforehand saturated with the toluene vapors. Experiments was carried out on two age groups (n=20): one and two months age rats. Inhalation period was 40 days, Each age group consisted of ten animals and was divided into the two subgroups: I - control animals (n=5), II - experimental animals (n=5). Assessment of the neurons' quantity in different structures was made according to the fractional approach method proposed by M. West. The results obtained showed that number of the pyramidal neurons decreases by 26% (I - 1067+/-83,8; II - 786+/-7; p<0,05) against the control in CA-3 field of the Subgroup II of the younger animals only. In the dentate fascia number of the granular cells does not change in either age group. Loss of pyramidal cells in the CA-3 field will induce deterioration of the hippocampal neural circuits and destroy of memory and learning processes in the rats.
在本研究中，分析了甲苯中毒对幼年和成年大鼠海马体中锥体细胞和颗粒细胞数量的影响。在我们的实验中采用吸入中毒途径——动物吸入甲苯直至达到侧卧姿势。该操作在封闭的玻璃容器中进行，其中空气预先用甲苯蒸汽饱和。实验在两个年龄组（n = 20）上进行：1个月龄和2个月龄的大鼠。吸入期为40天。每个年龄组由10只动物组成，并分为两个亚组：I——对照动物（n = 5），II——实验动物（n = 5）。根据M. West提出的分数法评估不同结构中神经元的数量。所得结果表明，仅在幼年动物亚组II的CA - 3区域中，锥体细胞数量相对于对照组减少了26%（I组——1067±83.8；II组——786±7；p<0.05）。在齿状回中，颗粒细胞数量在两个年龄组中均未发生变化。CA - 3区域中锥体细胞的缺失将导致大鼠海马神经回路的退化以及记忆和学习过程的破坏。
