Mione M C, Cavanagh J F, Lincoln J, Milner P, Burnstock G
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Cell Tissue Res. 1990 Mar;259(3):503-9. doi: 10.1007/BF01740777.
Using histochemical, immunohistochemical and biochemical techniques, noradrenaline-, neuropeptide Y-, vasoactive intestinal polypeptide-, substance P- and calcitonin gene-related peptide-containing nerve fibres were studied in the uterine artery of virgin, progesterone-treated and pregnant guinea-pigs. Morphological changes following hormone treatment or in pregnancy were also evaluated in a quantitative study on semithin sections of the uterine artery. In late pregnancy, the number of noradrenaline-containing nerve fibres, which formed the densest plexus in virgin animals, was significantly decreased, a finding supported by a significant reduction in noradrenaline levels. This reduction was not mimicked by systemic progesterone treatment. In contrast, the innervation of the uterine artery by neuropeptide Y-containing nerve fibres was increased in pregnancy, while the other peptidergic nerves and peptide levels were unchanged after progesterone treatment and in pregnancy. These changes led to a predominance of innervation by neuropeptide Y- rather than noradrenaline-containing nerve fibres in late pregnancy. No morphological changes were detected following progesterone treatment, but pregnancy led to a marked increase in the cross-sectional area of the vessel accompanied by an increase in the thickness of the media.
运用组织化学、免疫组织化学及生物化学技术，对未孕、经孕酮处理及怀孕豚鼠子宫动脉中含去甲肾上腺素、神经肽Y、血管活性肠肽、P物质及降钙素基因相关肽的神经纤维进行了研究。在对子宫动脉半薄切片的定量研究中，也评估了激素处理后或怀孕时的形态学变化。在妊娠后期，在未孕动物中形成最密集神经丛的含去甲肾上腺素神经纤维数量显著减少，去甲肾上腺素水平的显著降低也证实了这一发现。全身孕酮处理并未模拟出这种减少。相反，怀孕时含神经肽Y神经纤维对子宫动脉的神经支配增加，而其他肽能神经及肽水平在孕酮处理后及怀孕时未发生变化。这些变化导致妊娠后期神经肽Y而非含去甲肾上腺素神经纤维的神经支配占主导地位。孕酮处理后未检测到形态学变化，但怀孕导致血管横截面积显著增加，同时中膜厚度增加。
