Mione M C, Dhital K K, Amenta F, Burnstock G
Department of Anatomy and Developmental Biology, University College London, U.K.
Brain Res. 1988 Sep 13;460(1):103-13. doi: 10.1016/0006-8993(88)91210-3.
Perivascular nerve fibres containing noradrenaline (NA), serotonin (5-HT), substance P (SP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) were localized in whole-mount stretch preparations of the arteries of the rat circle of Willis using fluorescence and immunohistochemical techniques. Changes in the pattern and density of these perivascular nerves were studied from birth to 27 months of age. All perivascular nerve types reached a peak density of innervation at 1 month of age. This was followed by a general fall in the density of fluorescent nerve fibres. However, with aging, there was a decrease in the expression of vasoconstrictor neurotransmitters (NA and 5-HT) in cerebrovascular nerves, whereas the expression of vasodilator neurotransmitter (VIP and CGRP) in perivascular nerve fibres supplying the rat cerebral arteries was strikingly increased in old age. The density of NPY- and SP-containing nerve fibres was not significantly altered in old age. These changes are discussed in relation to the increased incidence of cerebrovascular disorders in the elderly.
采用荧光和免疫组织化学技术，在大鼠 Willis 环动脉的整装拉伸标本中定位了含有去甲肾上腺素（NA）、血清素（5-HT）、P 物质（SP）、血管活性肠肽（VIP）、神经肽 Y（NPY）和降钙素基因相关肽（CGRP）的血管周围神经纤维。研究了从出生到 27 个月龄这些血管周围神经的模式和密度变化。所有类型的血管周围神经在 1 月龄时达到神经支配密度峰值。随后荧光神经纤维密度普遍下降。然而，随着年龄增长，脑血管神经中血管收缩神经递质（NA 和 5-HT）的表达减少，而在老年大鼠脑动脉供应的血管周围神经纤维中，血管舒张神经递质（VIP 和 CGRP）的表达显著增加。老年时含 NPY 和 SP 的神经纤维密度无明显改变。结合老年人脑血管疾病发病率增加对这些变化进行了讨论。
