Pauley Kaleb M, Eystathioy Theophany, Jakymiw Andrew, Hamel John C, Fritzler Marvin J, Chan Edward K L
Department of Oral Biology, University of Florida, 1600 SW Archer Road, PO Box 100424, Gainesville, 32610-0424, USA.
EMBO Rep. 2006 Sep;7(9):904-10. doi: 10.1038/sj.embor.7400783. Epub 2006 Aug 11.
GW bodies (GWBs), or mammalian P bodies, proposed to be involved in messenger RNA storage and/or degradation, have recently been linked to RNA interference and microRNA (miRNA) processing. We report that endogenous let-7 miRNA co-precipitates with the GW182 protein complex. In addition, knockdown of two proteins, Drosha and its protein partner DGCR8, which are vital to the generation of mature miRNA, results in the loss of GWBs. Subsequent introduction of short interference RNA specific to lamin A/C is accompanied by reassembly of GWBs and concurrent knockdown of lamin A/C protein. Taken together, these studies show that miRNAs are crucial components in GWB formation.
GW小体（GWBs），即哺乳动物P小体，被认为参与信使核糖核酸（mRNA）的储存和/或降解，最近又与RNA干扰及微小核糖核酸（miRNA）加工联系在一起。我们报告称，内源性let-7 miRNA与GW182蛋白复合体共同沉淀。此外，对成熟miRNA生成至关重要的两种蛋白——Drosha及其蛋白伴侣DGCR8的敲低，会导致GW小体的缺失。随后引入针对核纤层蛋白A/C的短干扰RNA，会伴随着GW小体的重新组装以及核纤层蛋白A/C蛋白的同时敲低。综上所述，这些研究表明，miRNA是GW小体形成中的关键组成部分。
