Choi E K, Gatenby P A, Bateman J F, Cole W G
Clinical Immunology Centre, Royal Prince Alfred Hospital, Sydney, NSW, Australia.
Immunol Cell Biol. 1990 Feb;68 ( Pt 1):27-31. doi: 10.1038/icb.1990.4.
The role of autoimmunity to type II collagen in the arthritis of systemic lupus erythematosus (SLE) has been assessed by ELISA and by Western blotting cyanogen bromide cleavage fragments separated on SDS-PAGE. The results show that antibodies to both native and heat-denatured collagen are quite common in SLE when measured by ELISA. Of particular interest is the demonstration of an association between antibodies to the CB 11 peptide and deforming arthritis in SLE. This is the arthritogenic peptide in murine models of collagen II-induced autoimmune arthritis and the results presented here suggest a potential pathogenetic role in the deforming arthritis of SLE for this specific subset of antibodies to type II collagen.
通过酶联免疫吸附测定（ELISA）以及对在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上分离的溴化氰裂解片段进行蛋白质印迹法，评估了自身免疫对系统性红斑狼疮（SLE）关节炎中II型胶原的作用。结果表明，通过ELISA检测时，SLE患者中针对天然和热变性胶原的抗体相当常见。特别值得关注的是，在SLE中，针对CB 11肽的抗体与变形性关节炎之间存在关联得到了证实。这是II型胶原诱导的自身免疫性关节炎小鼠模型中的致关节炎肽，此处呈现的结果表明，这种特定的II型胶原抗体亚群在SLE的变形性关节炎中具有潜在的致病作用。
