Rowley M J, Mackay I R, Brand C A, Bateman J F, Chan D
Centre for Molecular Biology and Medicine, Monash University, Clayton, Victoria, Australia.
Rheumatol Int. 1992;12(2):65-9. doi: 10.1007/BF00300979.
Antibodies to human type II collagen were examined in the sera of 105 patients with rheumatoid arthritis (RA), 44 patients with systemic lupus erythematosus (SLE) and 11 patients who fulfilled the criteria of both diseases (RA-SLE overlap), using a solid-phase radioimmunoassay (RIA). The frequencies of antibodies to native and denatured human type II collagen were 20% and 27% in RA, 14% and 16% in SLE, and 45% and 36% in RA-SLE overlap. The specificity of the antibodies was further examined by inhibition with native and denatured type II collagen, by immunoblotting on native and denatured type II collagen, and by immunoblotting on cyanogen-bromide derived polypeptides of type II collagen. We could not identify any disease-specific patterns of reactivity. Thus, in the three disease groups the antibody response was polyclonal; there were antibody populations that reacted with native and/or denatured collagen, and epitopes could be assigned to at least three CB peptides, CB10.5, CB11 and CB8.
采用固相放射免疫分析法（RIA）检测了105例类风湿关节炎（RA）患者、44例系统性红斑狼疮（SLE）患者以及11例符合两种疾病标准（RA - SLE重叠）患者血清中的抗人Ⅱ型胶原抗体。RA患者中抗天然和变性人Ⅱ型胶原抗体的频率分别为20%和27%，SLE患者中分别为14%和16%，RA - SLE重叠患者中分别为45%和36%。通过用天然和变性Ⅱ型胶原进行抑制、对天然和变性Ⅱ型胶原进行免疫印迹以及对Ⅱ型胶原的溴化氰衍生多肽进行免疫印迹，进一步检测了抗体的特异性。我们未能识别出任何疾病特异性的反应模式。因此，在这三个疾病组中，抗体反应是多克隆的；存在与天然和/或变性胶原反应的抗体群体，并且表位可至少归属于三种CB肽，即CB10.5、CB11和CB8。
