Kao K J, Scornik J C, McQueen C F
Department of Pathology, University of Florida, Gainesville.
Hum Immunol. 1990 Apr;27(4):285-97. doi: 10.1016/0198-8859(90)90080-9.
In order to quantify each specific HLA-A or -B antigen on platelets, a monoclonal antibody against HLA heavy chains was developed and designated as 2F2 monoclonal antibody. This monoclonal antibody reacted on Western blot with platelet HLA from each of 10 individuals with different HLA phenotypes and precipitated all 35S-methionine-labeled HLA-A and -B antigens from three different Epstein-Barr Virus--transformed lymphoblastoid cell lines. The results indicate that the 2F2 monoclonal antibody recognizes an epitope shared by different HLA-A and -B antigens. The quantitative variation of specific HLA antigens on platelets was then studied in nine different donors by isoelectric-focusing gel electrophoresis and immunoblot using the 2F2 monoclonal antibody. The results of our studies showed that the shared HLA antigens such as A2, B35, and B62, varied three- to fivefold among different individuals and individual HLA-A or -B antigen was not equally expressed on a person's platelets. The relative quantities of specific HLA-A and -B antigens on lymphocytes were also noted to be the same as those on platelets. The finding suggests that differential expression of HLA specificities may not be restricted to platelets but is a more general phenomenon including other nucleated cells.
为了定量血小板上的每种特定HLA - A或 - B抗原，研发了一种针对HLA重链的单克隆抗体，并将其命名为2F2单克隆抗体。这种单克隆抗体在蛋白质印迹法中能与10名具有不同HLA表型个体的血小板HLA发生反应，并从三种不同的爱泼斯坦 - 巴尔病毒转化的淋巴母细胞系中沉淀出所有35S - 甲硫氨酸标记的HLA - A和 - B抗原。结果表明，2F2单克隆抗体识别不同HLA - A和 - B抗原共有的一个表位。然后，通过等电聚焦凝胶电泳和使用2F2单克隆抗体的免疫印迹法，在9名不同供体中研究了血小板上特定HLA抗原的定量变化。我们的研究结果显示，诸如A2、B35和B62等共有的HLA抗原在不同个体之间相差三到五倍，并且个体的HLA - A或 - B抗原在一个人的血小板上并非等量表达。淋巴细胞上特定HLA - A和 - B抗原的相对量也与血小板上的相同。这一发现表明，HLA特异性的差异表达可能并不局限于血小板，而是一种更普遍的现象，包括其他有核细胞。
