Martin T P, Gundersen L A, Vailas A C, Edgerton V R, Das S K
Faculty of Rehabilitation Medicine, University of Alberta, Edmonton, Canada.
J Appl Physiol (1985). 1990 Feb;68(2):687-92. doi: 10.1152/jappl.1990.68.2.687.
Skeletal muscle grafts performed with neurovascular repair are used extensively in clinical situations. However, most controlled experimental studies on the efficacy of such grafts have been conducted on muscles with a relatively small mass and over a limited recovery period. Therefore, selected cellular and matrix component properties of the comparatively large dog gracilis muscle (75 g) were studied 9-12 mo after orthotopic neurovascular grafting. The grafted muscle wet weights were 71% of the contralateral control (sham-operated) muscles. In addition, the concentrations of noncollagenous protein (13%), DNA (28%), and RNA (34%) were significantly reduced in the grafts. However, the concentration of collagen was significantly higher (41%) in the grafts. In this regard, the type III collagen phenotype showed the greatest relative increase. There was no difference between the grafted and control proteoglycan concentration. The metabolic profiles of the grafted muscles were significantly different from control. The activities of myofibrillar adenosinetriphosphatase (34%) and alpha-glycerophosphate dehydrogenase (25%) were reduced, whereas citrate synthase remained unchanged. These data suggest that recovery of up to 1 yr was insufficient for the normalization of several connective tissue matrix components and biochemical properties of the grafts.
带神经血管修复的骨骼肌移植在临床中被广泛应用。然而，大多数关于此类移植效果的对照实验研究都是在质量相对较小的肌肉上进行的，且恢复期有限。因此，在原位神经血管移植9至12个月后，对相对较大的犬股薄肌（75克）的选定细胞和基质成分特性进行了研究。移植肌肉的湿重为对侧对照（假手术）肌肉的71%。此外，移植肌肉中非胶原蛋白（13%）、DNA（28%）和RNA（34%）的浓度显著降低。然而，移植肌肉中胶原蛋白的浓度显著更高（41%）。在这方面，III型胶原蛋白表型的相对增加最大。移植肌肉和对照肌肉的蛋白聚糖浓度没有差异。移植肌肉的代谢谱与对照有显著不同。肌原纤维三磷酸腺苷酶（34%）和α-甘油磷酸脱氢酶（25%）的活性降低，而柠檬酸合酶保持不变。这些数据表明，长达1年的恢复期不足以使移植肌肉的几种结缔组织基质成分和生化特性恢复正常。
