Das S K, Spector S A, Miller T A, Martin T P, Edgerton V R
Plast Reconstr Surg. 1986 May;77(5):804-13. doi: 10.1097/00006534-198605000-00020.
Previous studies have suggested that successful transplantation of skeletal muscle to replace previously lost function depends on the mass of the transplanted tissue. In the present experiment, the possibility that careful microneurovascular surgical technique substantially improves the chances of successful transplantation of large-sized muscle was tested using dog gracilis muscle averaging 75 gm in weight. Gracilis muscles were completely excised ipsilaterally and were implanted into their original location (orthotopic) by reattaching tendons of insertion and origin. In addition, neurorrhaphies of nerve stumps were performed along with repair of the vascular pedicle using microsurgery techniques. After approximately 1 year, orthotopic transplants weighed about 70 percent of contralateral sham-operated gracilis muscles. Although average tension output of transplants declined to about 60 percent of control values, three of the most successfully transplanted muscles produced between 73 and 88 percent of control force. A significant increase in the number of slow-twitch-oxidative fibers was correlated with a slight but significant reduction in the maximal velocity of shortening of transplanted muscles. The ability of transplants to resist fatigue when repetitively stimulated was similar to the endurance capacity of control muscles. These results suggest that microneurovascular surgery may enhance the more complete restoration of function of transplanted skeletal muscles of relatively large size.
先前的研究表明，成功移植骨骼肌以替代先前丧失的功能取决于移植组织的质量。在本实验中，使用平均重量为75克的犬股薄肌，测试了精细的显微神经血管外科技术能否大幅提高大型肌肉成功移植的几率。股薄肌在同侧被完全切除，并通过重新连接起止肌腱植入其原位置（原位）。此外，使用显微外科技术进行神经断端神经缝合以及血管蒂修复。大约1年后，原位移植的肌肉重量约为对侧假手术股薄肌的70%。虽然移植肌肉的平均张力输出下降至对照值的约60%，但三块移植最成功的肌肉产生的力量为对照力的73%至88%。慢肌氧化纤维数量的显著增加与移植肌肉最大缩短速度的轻微但显著降低相关。移植肌肉在重复刺激时抵抗疲劳的能力与对照肌肉的耐力相似。这些结果表明，显微神经血管手术可能会增强相对大型移植骨骼肌功能的更完全恢复。
