Dey R D, Altemus J B, Zervos I, Hoffpauir J
Department of Anatomy, West Virginia University Health Sciences Center, Morgantown 26506.
J Appl Physiol (1985). 1990 Feb;68(2):770-8. doi: 10.1152/jappl.1990.68.2.770.
A combination of neuroanatomic techniques was used to examine the origin and neuropeptide content of nerve fibers in the airway epithelium of adult cats. By the use of immunocytochemical methods, the peptides substance P (SP) and calcitonin gene-related peptide (CGRP) were colocalized in airway epithelial nerve fibers. Two days after wheat germ agglutinin (WGA) was injected into the nodose ganglion, fibers containing WGA immunoreactivity (IR) were detected in the airway epithelium. SP-like immunoreactivity (LI) and CGRP-LI were demonstrated separately in the WGA-IR fibers, establishing their origin from nerve cell bodies of nodose ganglion. Vagal transection inferior to the nodose ganglion reduced the number of SP- and CGRP-IR fibers by greater than 90% in ipsilateral airways. In contralateral airways, SP-IR fibers were substantially reduced, whereas the effect on CGRP-IR fibers was not statistically significant. Vagotomy superior to the nodose ganglion did not alter the density of peptide-IR fibers. The results prove that SP- and CGRP-IR nerve fibers of cat airway epithelium originate from nerve cell bodies in the nodose ganglion and that SP- and CGRP-like peptides may be stored together in some nerve fibers of the airway epithelium.
运用多种神经解剖学技术，研究成年猫气道上皮神经纤维的起源和神经肽含量。通过免疫细胞化学方法，发现P物质（SP）和降钙素基因相关肽（CGRP）在气道上皮神经纤维中共定位。将小麦胚芽凝集素（WGA）注入结状神经节后两天，在气道上皮中检测到含有WGA免疫反应性（IR）的纤维。在WGA-IR纤维中分别显示出SP样免疫反应性（LI）和CGRP-LI，证实它们起源于结状神经节的神经细胞体。在结状神经节下方切断迷走神经，同侧气道中SP-IR和CGRP-IR纤维数量减少超过90%。在对侧气道中，SP-IR纤维显著减少，而对CGRP-IR纤维的影响无统计学意义。在结状神经节上方切断迷走神经，并未改变肽-IR纤维的密度。结果证明，猫气道上皮的SP-IR和CGRP-IR神经纤维起源于结状神经节的神经细胞体，并且SP样和CGRP样肽可能共同储存在气道上皮的一些神经纤维中。
