麻疹-腮腺炎-风疹三联疫苗效价测定的改进：各组分间的干扰及其消除措施。

Improvement in potency assay of measles-mumps-rubella trivalent vaccine: interference between components and measures for its elimination.

作者信息

Umino Y, Saito S, Fukuda A, Hishiyama M, Sugiura A

机构信息

Department of Measles Virus, National Institute of Health, Tokyo, Japan.

出版信息

J Virol Methods. 1990 Feb;27(2):159-68. doi: 10.1016/0166-0934(90)90132-y.

DOI:10.1016/0166-0934(90)90132-y

PMID:1690744

Abstract

In the potency assay of trivalent measles-mumps-rubella (MMR) vaccine by the immunocytochemical focus assay reported previously (Fukuda et al., 1987), development of rubella foci in RK13 cells was inhibited in the presence of a large excess of mumps component, resulting in an underestimation of the titre of the rubella component. When RK13 cells are infected with the mixture of mumps and rubella viruses, mumps virus interfered with the growth of rubella virus. Interference was mediated most likely by interferon induced by mumps virus. The interference was eliminated by a partial neutralization of mumps component by the addition of anti-mumps serum to the inoculum to RK13 cells. Improved method of potency assay of MMR vaccine incorporating the above measures and other modifications are described.

摘要

在先前报道的免疫细胞化学病灶分析法（Fukuda等人，1987年）对三价麻疹-腮腺炎-风疹（MMR）疫苗进行效价测定时，在存在大量过量腮腺炎成分的情况下，RK13细胞中风疹病灶的形成受到抑制，导致风疹成分的效价被低估。当RK13细胞感染腮腺炎病毒和风疹病毒的混合物时，腮腺炎病毒会干扰风疹病毒的生长。这种干扰很可能是由腮腺炎病毒诱导的干扰素介导的。通过向接种到RK13细胞的接种物中添加抗腮腺炎血清对腮腺炎成分进行部分中和，可消除这种干扰。本文描述了结合上述措施及其他改进的MMR疫苗效价测定的改进方法。