Grieder F B, Schultz K T
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
J Immunol. 1990 Apr 1;144(7):2627-31.
Syngeneic antiidiotypic (Anti-Id) mAb were produced to the Bluetongue virus (BTV) serotype 17 neutralizing mAb. Three anti-Id mAb had the characteristics of an internal image of the Id as demonstrated by 1) the internal image anti-Id mAb were capable of mimicking BTV by blocking the neutralizing activity of the idiotypic neutralizing mAb and 2) the anti-Id mAb bound specifically to the surface of BTV susceptible cells in indirect binding experiments as determined by immunocytochemistry and flow cytometric analysis. The potential application of these internal image anti-Id mAb in this arbovirus system is discussed.
制备了针对蓝舌病毒（BTV）17型中和单克隆抗体（mAb）的同基因抗独特型（Anti-Id）mAb。三种抗独特型mAb具有独特型内影像的特征，表现为：1）内影像抗独特型mAb能够通过阻断独特型中和mAb的中和活性来模拟BTV；2）通过免疫细胞化学和流式细胞术分析确定，在间接结合实验中，抗独特型mAb特异性结合BTV易感细胞表面。讨论了这些内影像抗独特型mAb在该虫媒病毒系统中的潜在应用。
