Mecham J O, Jochim M M
USDA, Agricultural Research Service, University Station, Laramie, WY.
Viral Immunol. 1990 Summer;3(2):161-70. doi: 10.1089/vim.1990.3.161.
Monoclonal antibodies were used to characterize neutralizing epitopes on VP2 of bluetongue virus serotype 10 (BTV-10). Six neutralizing monoclonal antibodies that immune precipitated VP2 demonstrated two distinct patterns of reactivity in the competitive enzyme-linked immune absorbent assay (ELISA). These results suggest that there are at least two distinct domains of neutralization on VP2 of BTV. Monoclonal antibodies defining the two domains were serotype-restricted in plaque neutralization, immune precipitation or ELISA. One of the two neutralizing domains also demonstrated significant hemagglutinating activity. Both cattle and sheep infected with BTV-10 produce antibodies to the two neutralizing epitopes.
单克隆抗体被用于鉴定蓝舌病毒10型（BTV-10）VP2上的中和表位。六种能免疫沉淀VP2的中和单克隆抗体在竞争性酶联免疫吸附测定（ELISA）中显示出两种不同的反应模式。这些结果表明，BTV的VP2上至少有两个不同的中和结构域。定义这两个结构域的单克隆抗体在蚀斑中和、免疫沉淀或ELISA中具有血清型限制性。两个中和结构域之一也显示出显著的血凝活性。感染BTV-10的牛和羊都会产生针对这两个中和表位的抗体。
