Kim G T, Webster G, Wimpenny J W T, Kim B H, Kim H J, Weightman A J
Cardiff School of Biosciences, Cardiff University, Cardiff, UK.
J Appl Microbiol. 2006 Sep;101(3):698-710. doi: 10.1111/j.1365-2672.2006.02923.x.
To characterize bacterial populations and their activities within a microbial fuel cell (MFC), using cultivation-independent and cultivation approaches.
Electron microscopic observations showed that the fuel cell electrode had a microbial biofilm attached to its surface with loosely associated microbial clumps. Bacterial 16S rRNA gene libraries were constructed and analysed from each of four compartments within the fuel cell: the planktonic community; the membrane biofilm; bacterial clumps (BC) and the anode biofilm. Results showed that the bacterial community structure varied significantly between these compartments. It was observed that Gammaproteobacteria phylotypes were present at higher numbers within libraries from the BC and electrode biofilm compared with other parts of the fuel cell. Community structure of the MFC determined by analyses of bacterial 16S rRNA gene libraries and anaerobic cultivation showed excellent agreement with community profiles from denaturing gradient gel electrophoresis (DGGE) analysis.
Members of the family Enterobacteriaceae, such as Klebsiella sp. and Enterobacter sp. and other Gammaproteobacteria with Fe(III)-reducing and electrochemical activity had a significant potential for energy generation in this system.
This study has shown that electrochemically active bacteria can be enriched using an electrochemical fuel cell.
采用非培养和培养方法，对微生物燃料电池（MFC）内的细菌群体及其活性进行表征。
电子显微镜观察显示，燃料电池电极表面附着有微生物生物膜，伴有松散结合的微生物团块。从燃料电池的四个隔室（浮游生物群落、膜生物膜、细菌团块（BC）和阳极生物膜）分别构建并分析细菌16S rRNA基因文库。结果表明，这些隔室之间的细菌群落结构存在显著差异。据观察，与燃料电池的其他部分相比，γ-变形菌门的系统发育型在来自BC和电极生物膜的文库中数量更多。通过细菌16S rRNA基因文库分析和厌氧培养确定的MFC群落结构与变性梯度凝胶电泳（DGGE）分析的群落图谱显示出极好的一致性。
肠杆菌科成员，如克雷伯菌属和肠杆菌属以及其他具有Fe(III)还原和电化学活性的γ-变形菌在该系统中具有显著的产电潜力。
本研究表明，可利用电化学燃料电池富集具有电化学活性的细菌。
