Kornbluth S, Cheng S H, Markland W, Fukui Y, Hanafusa H
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021-6399.
J Virol. 1990 Apr;64(4):1584-9. doi: 10.1128/JVI.64.4.1584-1589.1990.
A number of mutants of polyomavirus middle T antigen (MTag) were constructed into replication-competent avian retroviruses. To assess the ability of these MTag variants to transform and to associate with the avian p60c-src and p62c-yes proto-oncogene products, we used these viruses to infect chicken embryo fibroblasts. We found that the ability of individual mutant MTags to associate with p62c-yes correlated well with the ability of these mutants to transform, as has been previously shown for the association of MTag with p60c-src. All transformation-competent mutant MTags retained the ability to complex with p62c-yes. Two transformation-defective mutants, RX67 and RX68, which could weakly associate with p60c-src, were unable to associate with p62c-yes.dl1015, a transformation-defective mutant which could associate with p60c-src and with a phosphatidylinositol kinase activity, was also able to associate with p62c-yes. Therefore, some as yet unmeasured biochemical property is defective in this mutant.
将多瘤病毒中T抗原（MTag）的多个突变体构建到具有复制能力的禽逆转录病毒中。为了评估这些MTag变体转化以及与禽p60c-src和p62c-yes原癌基因产物结合的能力，我们用这些病毒感染鸡胚成纤维细胞。我们发现，单个突变型MTag与p62c-yes结合的能力与这些突变体的转化能力密切相关，正如之前所显示的MTag与p60c-src的结合情况一样。所有具有转化能力的突变型MTag都保留了与p62c-yes形成复合物的能力。两个转化缺陷型突变体RX67和RX68，它们与p60c-src的结合较弱，无法与p62c-yes结合。dl1015是一个转化缺陷型突变体，它能与p60c-src以及一种磷脂酰肌醇激酶活性结合，也能与p62c-yes结合。因此，这个突变体中某些尚未测定的生化特性存在缺陷。
