Ciccarelli C, Philipson L, Sorrentino V
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Mol Cell Biol. 1990 Apr;10(4):1525-9. doi: 10.1128/mcb.10.4.1525-1529.1990.
The suppression of growth arrest-specific (gas) gene expression by serum appeared to be independent of protein synthesis, but expression in resting cells was sensitive to 2-aminopurine, an inhibitor of intracellular protein kinases. Although accumulation of gas gene mRNA was reduced by serum, nuclear transcription of the gas-2, -3, and -5 genes was observed in serum-stimulated cells, indicating that posttranscriptional events may regulate mRNA levels. Growth induction by serum, on the other hand, led to suppression of transcription of the gas-1 gene. Cell cycle regulation and the serum response of gas-1 were lost in ras-transformed cells.
血清对生长停滞特异性（gas）基因表达的抑制似乎与蛋白质合成无关，但静止细胞中的表达对细胞内蛋白激酶抑制剂2-氨基嘌呤敏感。尽管血清可降低gas基因mRNA的积累，但在血清刺激的细胞中观察到gas-2、-3和-5基因的核转录，这表明转录后事件可能调节mRNA水平。另一方面，血清诱导生长导致gas-1基因转录受到抑制。在ras转化的细胞中，细胞周期调控和gas-1的血清反应丧失。
