Sako F, Gasa S, Makita A, Hayashi A, Nozawa S
Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Arch Biochem Biophys. 1990 Apr;278(1):228-37. doi: 10.1016/0003-9861(90)90252-t.
Two glycosphingolipids with human blood group A and H antigenicity were isolated from porcine erythrocyte membranes which were obtained from the pooled blood. The yield of the A- and H-antigenic glycolipids was approximately 0.2 and 0.1% of total neutral glycolipids, respectively. No B antigen was detected. Through several methods the porcine erythrocyte antigens were all found to belong to lactoseries (type 1 chain), IV2Fuc alpha, IV3GalNAc alpha Lc4Cer for type A and IV2-Fuc alpha Lc4Cer for type H, in contrast to the antigenic glycolipids in human erythrocytes, which mostly belong to neolactoseries (type 2 chain). The constituent fatty acids of the A antigen were 75% normal acids and 25% 2-hydroxy acids, and the long chain base was 95% sphingenine. This is the first demonstration of the A- and H-antigenic glycolipids on erythrocytes of pig in whose gastric mucin the human blood group A and H substances have been demonstrated.
从混合血液中获取的猪红细胞膜中分离出两种具有人血型A和H抗原性的糖鞘脂。A抗原性和H抗原性糖脂的产量分别约占总中性糖脂的0.2%和0.1%。未检测到B抗原。通过多种方法发现，猪红细胞抗原均属于乳糖系列（1型链），A型为IV2Fucα、IV3GalNAcαLc4Cer，H型为IV2-FucαLc4Cer，这与人类红细胞中的抗原性糖脂大多属于新乳糖系列（2型链）形成对比。A抗原的组成脂肪酸中75%为正常酸，25%为2-羟基酸，长链碱基95%为鞘氨醇。这是首次在猪的红细胞上证实A抗原性和H抗原性糖脂，此前已在猪胃粘蛋白中证实有人血型A和H物质。
