两种检测抗伯氏疏螺旋体抗体诊断试验的比较：基于单一基因种抗原的酶免疫分析和基于重组抗原的酶联免疫吸附测定
[Comparison of two types of diagnostic test detecting antibodies against Borrelia burgdorferi: EIA based on one genospecies antigens and ELISA based on recombinant antigens].
作者信息
Zajkowska Joanna, Kondrusik Maciej, Grygorczuk Sambor, Pancewicz Sławomir, Swierzbińska Renata, Sienkiewic Iwona, Hermanowska-Szpakowicz Teresa
机构信息
Klinika Chorób Zakaźnych i Neuroinfekcji, Akademii Medycznej w Białymstoku.
出版信息
Przegl Epidemiol. 2006;60 Suppl 1:171-6.
The aim of the study was to compare the results of ELISA diagnostic kit detecting antibodies against B. burgdorferi based on combination of three genospecies recombinant and EIA kits based on one of three genospecies. Sera of 351 forest workers were evaluated with ELISA kit (Recombinant antigen, IgG). Seropositive samples were tested with EIA kits based on B. burgdorferi s.s., B. garinii, B. afzelii antigens. Diagnostic kits based on combination of antigens of three genospecies more often detect antibodies against B. burgdorferi and are more usefull as screening tests, in comparison with those based on one genospecies. Among diagnostic kits based on one genospecies, the most sesnitive in detection of antibodies against B. burgdorferi are those based on B. afzelii.
该研究的目的是比较基于三种基因种重组体组合的ELISA诊断试剂盒检测抗伯氏疏螺旋体抗体的结果与基于三种基因种之一的EIA试剂盒的检测结果。使用ELISA试剂盒（重组抗原，IgG）对351名林业工人的血清进行了评估。血清学阳性样本用基于伯氏疏螺旋体、伽氏疏螺旋体、阿氏疏螺旋体抗原的EIA试剂盒进行检测。与基于单一基因种的诊断试剂盒相比，基于三种基因种抗原组合的诊断试剂盒更常检测到抗伯氏疏螺旋体抗体，作为筛查试验更有用。在基于单一基因种的诊断试剂盒中，检测抗伯氏疏螺旋体抗体最敏感的是基于阿氏疏螺旋体的试剂盒。
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