Thompson J, Koumari R, Wagner K, Barnert S, Schleussner C, Schrewe H, Zimmermann W, Müller G, Schempp W, Zaninetta D
Institute of Immunobiology, University of Freiburg, FRG.
Biochem Biophys Res Commun. 1990 Mar 16;167(2):848-59. doi: 10.1016/0006-291x(90)92103-7.
The pregnancy-specific glycoprotein (PSG) genes encode a group of proteins which are found in large amounts in placenta and maternal serum. In situ hybridization analyses of metaphase chromosomes reveal that all the human pregnancy-specific glycoprotein (PSG) genes are located on the long arm of chromosome 19 (19q13.2-13.3), overlapping the region containing the closely-related carcinoembryonic antigen (CEA) gene subgroup. Higher resolution analyses indicate that the PSG genes are closely linked within an 800kb SacII restriction endonuclease fragment. This has been confirmed through restriction endonuclease mapping and DNA sequence analyses of isolated genomic clones, which show that at least some of these genes are located in very close proximity. Further, these studies have helped to identify a new member of the PSG gene subfamily (PSG7). DNA/RNA hybridization analyses, using gene-specific oligonucleotide probes based on published sequences, showed that five from six PSG genes tested are coordinately transcribed in the placenta. Due to the close proximity of these genes and their coordinated expression pattern, common transcriptional regulatory elements may exist.
妊娠特异性糖蛋白（PSG）基因编码一组在胎盘和母体血清中大量存在的蛋白质。中期染色体的原位杂交分析表明，所有人类妊娠特异性糖蛋白（PSG）基因都位于19号染色体的长臂上（19q13.2 - 13.3），与包含密切相关的癌胚抗原（CEA）基因亚组的区域重叠。更高分辨率的分析表明，PSG基因在一个800kb的SacII限制性内切酶片段内紧密连锁。这已通过限制性内切酶图谱分析和分离的基因组克隆的DNA序列分析得到证实，这些分析表明其中至少一些基因位置非常接近。此外，这些研究有助于鉴定PSG基因亚家族的一个新成员（PSG7）。使用基于已发表序列的基因特异性寡核苷酸探针进行的DNA/RNA杂交分析表明，所测试的六个PSG基因中有五个在胎盘中协同转录。由于这些基因位置紧密且表达模式协同，可能存在共同的转录调控元件。
