Wojda Alina, Zietkiewicz Ewa, Mossakowska Małgorzata, Pawłowski Włodzimierz, Skrzypczak Krzysztof, Witt Michał
Institute of Human Genetics, Clinical and Molecular Genetics, Strzeszyńska 32, Poznań A 60-479, Poland.
J Gerontol A Biol Sci Med Sci. 2006 Aug;61(8):763-72. doi: 10.1093/gerona/61.8.763.
To answer whether the age-related accumulation of chromosomal damage differs in men and women, and whether the aberration level in centenarians is proportional to their age, cytogenetic aberrations in dividing cells were analyzed. G-band karyotyping of mitotic spreads from lymphocytes was performed in 52 Polish centenarians and 71 controls (aged 21-78). Statistical evaluation was performed using nonparametric tests and regression analysis. The average level of all chromosomal aberrations was comparable in centenarians of both genders, but the age-related increase in chromosomal damage occurred faster in women than in men. Aging in both genders was marked by the increasing level of all aberrations rather than by chromosome-specific changes; the loss of X chromosome was the leading contributor in women. The age-related increase in the level of chromosomal damage reflected accumulation of dividing cells with a small number of aberrations. Individuals who survive to the extreme old age appear to accumulate aberrations at the slower rate.
为了回答与年龄相关的染色体损伤积累在男性和女性中是否存在差异，以及百岁老人的畸变水平是否与其年龄成正比，我们对分裂细胞中的细胞遗传学畸变进行了分析。对52名波兰百岁老人和71名对照者（年龄在21 - 78岁之间）的淋巴细胞有丝分裂铺片进行了G带核型分析。使用非参数检验和回归分析进行统计评估。两性百岁老人中所有染色体畸变的平均水平相当，但女性染色体损伤随年龄的增加比男性更快。两性衰老的特征都是所有畸变水平的增加，而不是特定染色体的变化；X染色体的丢失是女性中畸变的主要原因。染色体损伤水平随年龄的增加反映了具有少量畸变的分裂细胞的积累。活到极高龄的个体似乎以较慢的速度积累畸变。
