Greif C, Hemmer O, Demangeat G, Fritsch C
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France.
J Gen Virol. 1990 Apr;71 ( Pt 4):907-15. doi: 10.1099/0022-1317-71-4-907.
Synthetic transcripts of tomato black ring virus satellite RNA (TBRV satRNA), isolate L, were prepared from cDNA cloned in the Bluescribe transcription vector. Transcripts with 49 (T49L) or two (T2GL) extra nucleotides at their 5' ends and 42 extra nucleotides at their 3' ends were able to induce, but to different extents, the synthesis in vitro of the satRNA-encoded 48K protein. However, when inoculated into Chenopodium quinoa together with TBRV L genomic RNAs, only T2GL was biologically active, in the presence or absence of a 5' cap analogue in the transcription reactions. Analysis of the 5' and 3' termini of the satRNA isolated from plants showed that nonviral extensions were not maintained in the transcript progeny.
番茄黑环病毒卫星RNA（TBRV satRNA）L分离株的合成转录本是从克隆于Bluescribe转录载体的cDNA制备而来。5'端带有49个（T49L）或两个（T2GL）额外核苷酸且3'端带有42个额外核苷酸的转录本能够在不同程度上诱导satRNA编码的48K蛋白的体外合成。然而，当与TBRV L基因组RNA一起接种到藜麦中时，无论转录反应中是否存在5'帽类似物，只有T2GL具有生物活性。对从植物中分离出的satRNA的5'和3'末端进行分析表明，转录后代中未保留非病毒延伸序列。
