Hans F, Fuchs M, Pinck L
Institut de Biologie Moléculaire des Plantes du CNRS, Laboratoire de Virologie, Strasbourg, France.
J Gen Virol. 1992 Oct;73 ( Pt 10):2517-23. doi: 10.1099/0022-1317-73-10-2517.
A set of full-length cDNA clones of the satellite RNA of grapevine fanleaf nepovirus isolate F13 (GFLV-F13) was constructed with a variable number of additional, non-viral nucleotides at the 5' and 3' ends. The biological activity of the RNAs transcribed from these constructs was tested in Chenopodium quinoa protoplasts using a helper virus. When inoculated with arabis mosaic virus S (ArMV-S) RNA as helper, transcripts with 33 non-viral nucleotides at the 5' end (tr45p4) did not replicate, whereas transcripts with only one non-viral nucleotide at the 5' end (tr3S and tr3M) did replicate. Capping of the transcripts enhanced their replication. On the other hand, the presence of extra nucleotides at the 3' end had little influence on the biological activity of the in vitro transcripts. In contrast with ArMV-S, GFLV isolate 24 was not a helper for tr3M transcripts, indicating a specific interaction between the helper strain and the satellite RNA.
构建了葡萄扇叶病毒分离株F13（GFLV - F13）卫星RNA的一组全长cDNA克隆，其5'和3'末端带有可变数量的额外非病毒核苷酸。使用辅助病毒在藜麦原生质体中测试了从这些构建体转录的RNA的生物学活性。当以阿拉伯花叶病毒S（ArMV - S）RNA作为辅助病毒接种时，5'末端带有33个非病毒核苷酸的转录本（tr45p4）不复制，而5'末端仅带有一个非病毒核苷酸的转录本（tr3S和tr3M）则复制。转录本的加帽增强了它们的复制。另一方面，3'末端额外核苷酸的存在对体外转录本的生物学活性影响很小。与ArMV - S相反，GFLV分离株24不是tr3M转录本的辅助病毒，表明辅助病毒株与卫星RNA之间存在特异性相互作用。
