Swanson P E, Wick M R
Department of Laboratory Medicine and Pathology, University of Minnesota School of Medicine.
Mod Pathol. 1990 Mar;3(2):113-9.
Recent studies of Class II histocompatibility antigen expression in bone and soft tissue sarcomas have suggested that malignant fibrous histiocytoma (MFH) may express HLA-DR, whereas histologically similar pleomorphic, epithelioid, and spindle cell malignant neoplasms generally do not. To test whether these observations are reproducible in the differential diagnosis of soft tissue sarcomas, anti-HLA-DR antibodies LK8D3 and LN3 were applied to formalin-fixed, paraffin-embedded sections of MFH, neurofibrosarcoma (NFS), leiomyosarcoma (LMS), synovial sarcoma (SS), fibrosarcoma (FS), angiosarcoma (AS), Kaposi's sarcoma (KS), chondrosarcoma (ChS), "dedifferentiated" chondrosarcoma (DChS), osteosarcoma (OS), epithelioid sarcoma (ES), and clear cell sarcoma (CCS; malignant melanoma of soft parts). The only consistent difference in Class II antigen expression was seen in the group of neoplasms composed of large polygonal cells. Among the latter lesions, four of six clear cell sarcomas were labeled by LK8D3 or LN3, but none of 12 epithelioid sarcomas were reactive. Otherwise, a diversity of tumors in other morphologic categories expressed Class II antigens, with no clear diagnostic patterns. These results may be of use in the diagnostic separation of large cell epithelioid tumors of soft tissue, but neither LN3 nor LK8D3 appears to be helpful in the identification of other sarcomas.
近期关于骨与软组织肉瘤中II类组织相容性抗原表达的研究表明，恶性纤维组织细胞瘤（MFH）可能表达HLA - DR，而组织学上类似的多形性、上皮样和梭形细胞恶性肿瘤通常不表达。为了检验这些观察结果在软组织肉瘤的鉴别诊断中是否可重复，将抗HLA - DR抗体LK8D3和LN3应用于MFH、神经纤维肉瘤（NFS）、平滑肌肉瘤（LMS）、滑膜肉瘤（SS）、纤维肉瘤（FS）、血管肉瘤（AS）、卡波西肉瘤（KS）、软骨肉瘤（ChS）、“去分化”软骨肉瘤（DChS）、骨肉瘤（OS）、上皮样肉瘤（ES）和透明细胞肉瘤（CCS；软组织恶性黑色素瘤）的福尔马林固定、石蜡包埋切片。II类抗原表达中唯一一致的差异见于由大的多边形细胞组成的肿瘤组。在后者的病变中，6例透明细胞肉瘤中有4例被LK8D3或LN3标记，但12例上皮样肉瘤均无反应。否则，其他形态学类别的多种肿瘤表达II类抗原，无明确的诊断模式。这些结果可能有助于软组织大细胞上皮样肿瘤的诊断鉴别，但LN3和LK8D3似乎都无助于其他肉瘤的鉴别。
